Isoguanine quartets formed by d(T(4)isoG(4)T(4)): Tetraplex identification and stability

被引：59

作者：

Seela, F

Wei, CF

Melenewski, A

机构：

[1] Lab. F. Org. und Bioorganische Chem., Institut für Chemie, Universität Osnabrück, D-49069 Osnabrück

来源：

NUCLEIC ACIDS RESEARCH | 1996年 / 24卷 / 24期

关键词：

D O I：

10.1093/nar/24.24.4940

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The self-aggregation of the oligonucleotide d(T(4)isoG(4)T(4)) (1) is investigated. Based on ion exchange HPLC experiments and CD spectroscopy, a tetrameric structure is identified, This structure was formed in the presence of sodium ions and shows almost the same chromatographic mobility on ion exchange HPLC as d(T(4)G(4)T(4)) (2) The ratio of aggregate versus monomer is temperature dependent and the tetraplex of [d(T(4)isoG(4)T(4))](4) is more stable than that of [d(T(4)G(4)T(4))](4) A mixture of d(T(4)isoG(4)T(4)) and d(T(4)G(4)T(4)) forms mixed tetraplexes containing strands of d(T(4)isoG(4)T(4)) and d(T(4)G(4)T(4)).

引用

页码：4940 / 4945

页数：6

共 24 条

[1] THE MOLECULAR-STRUCTURE OF CENTROMERES AND TELOMERES [J].