Coumarinic acid-based cyclic prodrugs of opioid peptides that exhibit metabolic stability to peptidases and excellent cellular permeability

被引:51
作者
Gudmundsson, OS
Pauletti, GM
Wang, W
Shan, DX
Zhang, HJ
Wang, BH
Borchardt, RT [1 ]
机构
[1] Univ Kansas, Dept Pharmaceut Chem, Lawrence, KS 66047 USA
[2] N Carolina State Univ, Dept Chem, Raleigh, NC 27695 USA
关键词
esterase-sensitive prodrugs; peptide delivery; opioid peptides; Caco-2; cells; membrane permeability; chemical and enzymatic stability;
D O I
10.1023/A:1018828207920
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Purpose. To evaluate the cellular permeation characteristics and the chemical and enzymatic stability of coumarinic acid-based cyclic prodrugs (1) under bar and (2) under bar of the opioid peptides [Leu(5)]-enkephalin (H-Tyr-Gly-Gly-Phe-Leu-OH) and DADLE (H-Tyr-D-Ala-Gly-Phe-D-Leu-OH), respectively. Methods. The rates of conversion of the cyclic prodrugs (1) under bar and (2) under bar to [Leu(5)]-enkephalin and DADLE, respectively, in HBSS, pH 7.4 (Caco-2 cell transport buffer) and in various biological media having measurable esterase activity were determined by HPLC. The cell permeation characteristics of [Leu(5)]-enkephalin, DADLE and cyclic prodrugs (1) under bar and (2) under bar were measured using Caco-2 cell monolayers grown onto microporus membranes and monitored by HPLC. Results. In HBSS, pH 7.4, cyclic prodrugs (1) under bar and (2) under bar degraded chemically to intermediates that further degraded to [Leu(5)]-enkephalin and DADLE, respectively, in stoichiometric amounts. In 90% human plasma and rat liver homogenate, the disappearance of cyclic prodrugs (1) under bar and (2) under bar was significantly faster than in HBSS, pH 7.4. The half-lives in 90% human plasma and in rat liver homogenate were substantially longer after pretreatment with paraoxon, a known inhibitor of serine-dependent esterases. When applied to the AP side of a Caco-2 cell monolayer, cyclic prodrug (1) under bar exhibited significantly greater stability against peptidase metabolism than did [Leu(5)]-enkephalin, Cyclic prodrug (2) under bar and DADLE exhibited similar stability when applied to the AP side of the Caco-2 cell monolayer. Prodrug (1) under bar was 665-fold more able to permeate the Caco-2 cell monolayers than was [Leu(5)]-enkephalin, in part because of its increased enzymatic stability. Prodrug (2) under bar was shown to be approximately 31 fold more able to permeate a Caco-2 cell monolayer than was DADLE. Conclusions. Cyclic prodrugs (1) under bar and (2) under bar, prepared with the coumarinic acid promoiety, were substantially more able to permeate Caco-2 cell monolayers than were the corresponding opioid peptides. Prodrug (1) under bar exhibited increased stability to peptidase metabolism compared to [Leu(5)]-enkephalin. In various biological media, the opioid peptides were released from the prodrugs by an esterase-catalyzed reaction, which is sensitive to paraoxon inhibition.
引用
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页码:7 / 15
页数:9
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