Structural characterization of an immunoregulatory polysaccharide from the fruiting bodies of Lepista sordida

A water-soluble polysaccharide, named as LSP, was extracted and fractioned from the fruiting bodies of Lepista sordida by DEAE-cellulose anion exchange and Sepharose CL-6B column chromatography. Its molecular weight (Mw) was estimated to be about 4 x 10(4) Da by using high-performance gel permeation chromatography (HPGPC). According to Fourier transform infrared (FT-IR) spectroscopy, partial acid hydrolysis, periodate oxidation and Smith degradation, methylation and GC-MS analysis, the results indicated LSP had a backbone consisting of (1 -> 6)-linked-alpha-D-glucopyranosyl and (1 -> 2,6)-linked-alpha-D-glucopyranosyl residues, which was terminated with terminal (1 -> )-alpha-D-galactopyranosyl residue at the O-3 position of (1 -> 2,6)-linked-alpha-D-glucopyranosyl residue along the main chain in the ratio of 2:1.1:0.9. Preliminary tests in vitro showed LSP had potent activation effects on murine macrophages, supported by the fact that the elevated NO production and TNF-alpha secretion, and its branches played a crucial role for macrophages activation. (C) 2012 Elsevier Ltd. All rights reserved.