Comparison of human and Xenopus GATA-2 promoters

被引：15

作者：

Fleenor, DE

Langdon, SD

deCastro, CM

Kaufman, RE

机构：

[1] Duke University Medical Center, Box 3250, Department of Medicine, Durham

来源：

GENE | 1996年 / 179卷 / 02期

关键词：

ets factors; CCAAT factors; gene; cloning; erythropoiesis;

D O I：

10.1016/S0378-1119(96)00355-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

GATA proteins comprise a family of transcription factors that are required for appropriate development of hematopoietic lineages. In order to understand the transcriptional regulation of GATA genes, we have cloned the human GATA-2 gene and identified and characterized its promoter. Comparison with the Xenopus GATA-2 promoter demonstrates highly conserved CCAAT box elements, which are essential for appropriate Xenopus expression. Unlike the Xenopus gene, the human GATA-2 gene lacks GATA binding motifs within the first 800 bp of 5' flanking sequence. In addition, the human GATA-2 promoter has two highly conserved ets sites that resemble the binding site for a recently described ets repressor factor, ERF. These conserved DNA sequence motifs provide strong candidate regions for the regulation of GATA-2.

引用

页码：219 / 223

页数：5

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