Acylspermidine derivatives isolated from a soft coral, Sinularia sp., inhibit plant vacuolar H+-pyrophosphatase

被引:12
作者
Hirono, M
Ojika, M
Mimura, H
Nakanishi, Y
Maeshima, M [1 ]
机构
[1] Nagoya Univ, Sch Bioagr Sci, Lab Cell Dynam, Nagoya, Aichi 4648601, Japan
[2] Nagoya Univ, Sch Bioagr Sci, Lab Nat Prod & Mol Funct Modeling, Nagoya, Aichi 4648601, Japan
关键词
acylspermidine; H+-pyrophosphatase; inhibitor; soft coral; vacuole;
D O I
10.1093/jb/mvg103
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
H+-pyrophosphatase (H+-PPase), which pumps H+ across membranes coupled with PPi hydrolysis, is found in most plants, and some parasitic protists, eubacteria and archaebacteria. We assayed a number of extracts derived from 145 marine invertebrates as to their inhibitory effect on plant vacuolar H+-PPase. Acylspermidine derivatives [RCONH(CH2)(3)N(CH3)(CH2)(4)N(CH3)(2)] from a soft coral (Sinularia sp.) inhibited the PPi-hydrolysis activity of purified H+-PPase and the PPi-dependent H+ pump activity (half inhibition concentration, 1 muM) of vacuolar membranes of mung bean. The apparent K-i was determined to be 0.9 muM. Acylspermidines did not affect the activity of vacuolar H+-ATPase, plasma membrane H+-ATPase, mitochondrial ATPase or cytosolic PPase. Acylspermidines inhibited the acidification of vacuoles in protoplasts, as found on monitoring by the acridine orange fluorescent method. These results indicate that acylspermidine derivatives represent new inhibitors of H+-PPase with relatively high specificity.
引用
收藏
页码:811 / 816
页数:6
相关论文
共 28 条
[1]   Calcium regulation in the intraerythrocytic malaria parasite Plasmodium falciparum [J].
Alleva, LM ;
Kirk, K .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2001, 117 (02) :121-128
[2]  
[Anonymous], 1979, PROG CHEM
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   Cytotoxic acylated spermidine from a soft coral, Sinularia sp. [J].
Choi, YH ;
Schmitz, FJ .
JOURNAL OF NATURAL PRODUCTS, 1997, 60 (05) :495-496
[5]   Acidocalcisome:: A novel Ca2+ storage compartment in trypanosomatids and apicomplexan parasites [J].
Docampo, R ;
Moreno, SNJ .
PARASITOLOGY TODAY, 1999, 15 (11) :443-448
[6]   Vacuolar H+ pyrophosphatases:: from the evolutionary backwaters into the mainstream [J].
Drozdowicz, YM ;
Rea, PA .
TRENDS IN PLANT SCIENCE, 2001, 6 (05) :206-211
[7]   Marine natural products [J].
Faulkner, DJ .
NATURAL PRODUCT REPORTS, 2002, 19 (01) :1-48
[8]  
Fenical W., 1978, MARINE NATURAL PRODU, VII, P173
[9]   SPERMIDINE DERIVATIVES AND 9,11-SECOSTEROIDS FROM A SOFT CORAL (SINULARIA SP) [J].
KAZLAUSKAS, R ;
MURPHY, PT ;
RAVI, BN ;
SANDERS, RL ;
WELLS, RJ .
AUSTRALIAN JOURNAL OF CHEMISTRY, 1982, 35 (01) :69-75
[10]   Membrane transport in the malaria-infected erythrocyte [J].
Kirk, K .
PHYSIOLOGICAL REVIEWS, 2001, 81 (02) :495-537