Two EST-derived marker systems for cultivar identification in tree peony

被引：69

作者：

Zhang, J. J. ^{[1
,2
]}

Shu, Q. Y. ^{[1
]}

Liu, Z. A. ^{[1
]}

Ren, H. X. ^{[1
]}

Wang, L. S. ^{[1
]}

De Keyser, E. ^{[3
]}

机构：

[1] Chinese Acad Sci, Beijing Bot Garden, Inst Bot, Beijing 100093, Peoples R China

[2] Minist Agr, Human Resources Dev Ctr, Beijing 100125, Peoples R China

[3] Appl Genet & Breeding Inst Agr & Fisheries Res, Plant Sci Unit, B-9090 Melle, Belgium

来源：

PLANT CELL REPORTS | 2012年 / 31卷 / 02期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

Tree Peony; TRAP; EST-SSR; Fingerprinting; REGION AMPLIFICATION POLYMORPHISM; SSR-MARKERS; GENETIC DIVERSITY; TRANSFERABILITY; TRAP; DATABASES; WHEAT; QTL; DNA;

D O I：

10.1007/s00299-011-1164-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Tree peony (Paeonia suffruticosa Andrews), a woody deciduous shrub, belongs to the section Moutan DC. in the genus of Paeonia of the Paeoniaceae family. To increase the efficiency of breeding, two EST-derived marker systems were developed based on a tree peony expressed sequence tag (EST) database. Using target region amplification polymorphism (TRAP), 19 of 39 primer pairs showed good amplification for 56 accessions with amplicons ranging from 120 to 3,000 bp long, among which 99.3% were polymorphic. In contrast, 7 of 21 primer pairs demonstrated adequate amplification with clear bands for simple sequence repeats (SSRs) developed from ESTs, and a total of 33 alleles were found in 56 accessions. The similarity matrices generated by TRAP and EST-SSR markers were compared, and the Mantel test (r = 0.57778, P = 0.0020) showed a moderate correlation between the two types of molecular markers. TRAP markers were suitable for DNA fingerprinting and EST-SSR markers were more appropriate for discriminating synonyms (the same cultivars with different names due to limited information exchanged among different geographic areas). The two sets of EST-derived markers will be used further for genetic linkage map construction and quantitative trait locus detection in tree peony.

引用

页码：299 / 310

页数：12

共 40 条

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Target region amplification polymorphism (TRAP) for assessing genetic diversity in sugarcane germplasm collections [J].