Expression of Fas ligand by microglia: possible role in glioma immune evasion

被引：87

作者：

Badie, B ^{[1
]}

Schartner, J ^{[1
]}

Prabakaran, S ^{[1
]}

Paul, J ^{[1
]}

Vorpahl, J ^{[1
]}

机构：

[1] Univ Wisconsin, Sch Med, Clin Sci Ctr K3 805, Dept Neurol Surg,Neurooncol Lab, Madison, WI 53792 USA

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2001年 / 120卷 / 1-2期

关键词：

brain; monocytes/macrophages; immunomodulation; neuroimmunology; tumor immunity;

D O I：

10.1016/S0165-5728(01)00361-7

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The immune-privileged status of the central nervous system is thought to limit the application of immunotherapy for treatment of malignant brain tumors. Because the Fas pathway has been proposed to play a role in immune evasion, we examined the effect of tumor environment on the expression of Fas ligand (FasL) in a mouse glioma model. Immunoblotting revealed the expression of membrane-bound FasL to nearly double when murine G26 gliomas were propagated intracranially (IC) as compared to subcutaneously (SC). Further analysis by flow cytometry revealed microglia, which were absent in the SC tumors, to account for half of the FasL expression in the IC tumors. Interestingly, when FasL activity was inhibited in IC tumors, the proportion of tumor-infiltrating leukocytes increased three-fold, reaching the same frequency as the SC tumors. These observations suggest that microglia are a major source of FasL expression in brain rumors and possibly contribute to the local immunosuppressive milieu of malignant gliomas. (C) 2001 Elsevier Science BN. All rights reserved.

引用

页码：19 / 24

页数：6

共 27 条

[11] Modulation of Fas-Ligand (Fas-L) on human microglial cells: an in vitro study [J].