Starch deposition and amylase accumulation during somatic embryogenesis in bamboo (Dendrocalamus hamiltonii)

被引:22
作者
Godbole, S [1 ]
Sood, A [1 ]
Sharma, M [1 ]
Nagar, PK [1 ]
Ahuja, PS [1 ]
机构
[1] Inst HImalayan Bioresource Technol, Div Biotechnol, Palampur 176061, Himachal Prades, India
关键词
amylase; bamboo; Dendrocalamus hamiltonii; scutellum; somatic embryo; starch deposition;
D O I
10.1078/0176-1617-01219
中图分类号
Q94 [植物学];
学科分类号
071001 [植物学];
摘要
In monocots, the zygotic embryo is protected and nourished by an endosperm. In the present study starch deposition and amylase accumulation was noticed during somatic embryogenesis in stem callus of a bamboo, Dendrocalamus hamiltonii. SEM studies revealed that starch grains were clearly visible in the scutellum during the maturation stage of the somatic embryo. As the somatic embryo developed further, the scutellum got reduced with corresponding increase in amylase. The amylase activity was tested periodically at different developmental stages of embryos. The role of scutellum in somatic embryos for starch deposition and amylase accumulation is discussed.
引用
收藏
页码:245 / 248
页数:4
相关论文
共 13 条
[1]
Hormonal signalling in cereal aleurone [J].
Bethke, PC ;
Schuurink, R ;
Jones, RL .
JOURNAL OF EXPERIMENTAL BOTANY, 1997, 48 (312) :1337-1356
[2]
Godbole S, 2002, CURR SCI INDIA, V83, P885
[3]
The alpha-amylase multigene family [J].
Mitsui, T ;
Itoh, K .
TRENDS IN PLANT SCIENCE, 1997, 2 (07) :255-261
[4]
Polymorphism in rice amylases at an early stage of seed germination [J].
Mitsunaga, S ;
Kawakami, O ;
Numata, T ;
Yamaguchi, J ;
Fukui, K ;
Mitsui, T .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2001, 65 (03) :662-665
[5]
A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES [J].
MURASHIGE, T ;
SKOOG, F .
PHYSIOLOGIA PLANTARUM, 1962, 15 (03) :473-497
[8]
SHUSTER L, 1962, ARCH BIOCHEM BIOPHYS, V96, P535
[9]
Sood A., 1994, BIOTECHNOLOGY INDIA, P199
[10]
A QUANTITATIVE ASSESSMENT OF THE IMPORTANCE OF BARLEY SEED ALPHA-AMYLASE, BETA-AMYLASE, DEBRANCHING ENZYME, AND ALPHA-GLUCOSIDASE IN STARCH DEGRADATION [J].
SUN, ZT ;
HENSON, CA .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1991, 284 (02) :298-305