In vitro mutagenicity assessment of aluminium oxide nanomaterials using the Salmonella/microsome assay

被引：41

作者：

Balasubramanyam, A. ^{[1
]}

Sailaja, N. ^{[1
]}

Mahboob, M. ^{[1
]}

Rahman, M. F. ^{[1
]}

Hussain, Saber M. ^{[2
]}

Grover, Paramjit ^{[1
]}

机构：

[1] Indian Inst Chem Technol, Div Biol, Toxicol Unit, Hyderabad 500607, Andhra Pradesh, India

[2] USAF, Res Lab, HEPB, Wright Patterson AFB, OH 45431 USA

来源：

TOXICOLOGY IN VITRO | 2010年 / 24卷 / 06期

关键词：

Nanomaterials; Aluminium oxide; Ames test; Salmonella; Mutagenicity; ULTRAFINE TIO2 PARTICLES; WALLED CARBON NANOTUBES; NANOPARTICLES; GENOTOXICITY; NANOFIBRES; DISPERSION; TOXICITY;

D O I：

10.1016/j.tiv.2010.07.004

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 [卫生毒理学];

摘要：

The aim of the current study was to evaluate the potential mutagenicity of aluminium oxide nanomaterials (NMs) (Al2O3-30 nm and Al2O3-40 nm). Characterization of the NMs was done before the initiation of the study. The mutagenicity of the NMs was studied by the Ames test with Salmonella typhimurium TA100, TA1535, TA98, TA97a and TA102 strains, in the presence and absence of the S9 mixture. Based on a preliminary cytotoxicity study conducted on the strains, different concentrations of Al2O3-30 nm, Al2O3-40 nm and Al2O3-bulk were selected. At all the concentrations tested. Al2O3-30 nm and Al2O3-40 nm did not significantly increase the number of revertant colonies compared to the Al2O3-bulk and control with or without S9 mixture. Our findings suggest that Al2O3 NMs were devoid of any size and concentration dependent mutagenicity compared to the Al2O3-bulk and control. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1871 / 1876

页数：6

共 38 条

[1]

METHODS FOR DETECTING CARCINOGENS AND MUTAGENS WITH SALMONELLA-MAMMALIAN-MICROSOME MUTAGENICITY TEST [J].

AMES, BN ;

MCCANN, J ;

YAMASAKI, E .

MUTATION RESEARCH, 1975, 31 (06) :347-363

[2]

[Anonymous], OECD GUID TEST CHEM

[3]

Evaluation of genotoxic effects of oral exposure to Aluminum oxide nanomaterials in rat bone marrow [J].

Balasubramanyam, A. ;

Sailaja, N. ;

Mahboob, M. ;

Rahman, M. F. ;

Misra, S. ;

Hussain, Saber M. ;

Grover, Paramjit .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2009, 676 (1-2) :41-47

[4]

In vivo genotoxicity assessment of aluminium oxide nanomaterials in rat peripheral blood cells using the comet assay and micronucleus test [J].

Balasubramanyam, A. ;

Sailaja, N. ;

Mahboob, M. ;

Rahman, M. F. ;

Hussain, Saber M. ;

Grover, Paramjit .

MUTAGENESIS, 2009, 24 (03) :245-251

[5]

*DEP DEF DIR, 2005, DEF RES ENG 2005

[6]

Multi-walled carbon nanotubes: Lack of mutagenic activity in the bacterial reverse mutation assay [J].

Di Sotto, Antonella ;

Chiaretti, Massimo ;

Carru, Giovanna Angela ;

Bellucci, Stefano ;

Mazzanti, Gabriela .

TOXICOLOGY LETTERS, 2009, 184 (03) :192-197

[7]

Nanotoxicology [J].

Donaldson, K ;

Stone, V ;

Tran, CL ;

Kreyling, W ;

Borm, PJA .

OCCUPATIONAL AND ENVIRONMENTAL MEDICINE, 2004, 61 (09) :727-728

[8]

Clastogenicity, photo-clastogenicity or pseudo-photo-clastogenicity: Genotoxic effects of zinc oxide in the dark, in pre-irradiated or simultaneously irradiated Chinese hamster ovary cells [J].

Dufour, Eric K. ;

Kumaravel, Tirukalikundram ;

Nohynek, Gerhard J. ;

Kirkland, David ;

Toutain, Herve .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2006, 607 (02) :215-224

[9]

HUNLEY JD, 1999, JOINT PROP C EXH HEL

[10]

Characterization of size, surface charge, and agglomeration state of nanoparticle dispersions for toxicological studies [J].

Jiang, Jingkun ;

Oberdorster, Gunter ;

Biswas, Pratim .

JOURNAL OF NANOPARTICLE RESEARCH, 2009, 11 (01) :77-89

← 1 2 3 4 →