A modified catalase assay suitable for a plate reader and for the analysis of brain cell cultures

被引：48

作者：

Cohen, G ^{[1
]}

Kim, M ^{[1
]}

Ogwu, V ^{[1
]}

机构：

[1] CUNY MT SINAI SCH MED, NEUROBIOL RES CTR, NEW YORK, NY 10029 USA

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1996年 / 67卷 / 01期

关键词：

catalase assay; cell culture; plate reader; mesencephalon;

D O I：

10.1016/S0165-0270(96)00011-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzyme catalase is present in relatively small amounts in neural tissue. A standard spectrophotometric assay for catalase has been modified to make it suitable both for automated assay with a plate reader acid for the analysis of neural cell cultures. Catalase activity is determined by measuring residual hydrogen peroxide after incubation with the enzyme. Ferrous ions and thiocyanate are used for the spectrophotometric determination of hydrogen peroxide. The stable visible absorption of ferrithiocyanate can be measured either with a plate reader or a spectrophotometer. The method assays catalase activity from single wells of a tissue culture plate containing 2-3 mg of embryonic rat mesencephalon.

引用

页码：53 / 56

页数：4

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