Specific interaction of oxidized low-density lipoprotein with macrophage-derived foam cells isolated from rabbit atherosclerotic lesions

被引：28

作者：

de Vries, HE ^{[1
]}

Buchner, B ^{[1
]}

van Berkel, TJC ^{[1
]}

Kuiper, J ^{[1
]}

机构：

[1] Leiden Univ, Sylvius Labs, Leiden Amsterdam Ctr Drug Res, Div Biopharmaceut, NL-2333 AL Leiden, Netherlands

来源：

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY | 1999年 / 19卷 / 03期

关键词：

scavenger receptor; oxidized lipoproteins; macrophage-derived foam cells; monocytes;

D O I：

10.1161/01.ATV.19.3.638

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Interaction of oxidized LDL (OxLDL) with macrophage-derived foam cells is one of the key events in the development and progression of atherosclerosis. To study this interaction, macrophage-derived foam cells were isolated from rabbit atherosclerotic lesions and the expression of scavenger receptors for OxLDL was examined. Atherosclerosis was induced in rabbits by denudation of the large arteries, followed by a hypercholesteremic diet. Macrophage-derived foam cells, characterized by immunostaining with an RAM-11 antibody (a macrophage marker), contained a high content of intracellular Lipid. Maximal binding of radiolabeled OxLDL to isolated macrophage-derived foam cells (1652+/-235 ng I-125-OxLDL/mg of cell protein) was 20-fold higher compared with B-max values of monocytes. Levels of association of OxLDL to macrophage-derived foam cells isolated from atherosclerotic lesions 12 weeks after denudation were >3-fold higher compared with the levels expressed by macrophage-derived foam cells isolated after 6 weeks. Association of I-125-OxLDL could be completely blocked by OxLDL, and partially by acetylated LDL and polyinosinic acid, indicating the presence of a specific binding site for OxLDL on macrophage-derived foam cells. The induction of scavenger receptors for OxLDL on macrophage-derived foam cells during the development of atherosclerosis, as described in this study, may facilitate the lipid accumulation in macrophage-derived foam cells, as observed in advanced atherosclerotic lesions.

引用

页码：638 / 645

页数：8

共 44 条

[21] REGULATION OF SMOOTH-MUSCLE CELL SCAVENGER RECEPTOR EXPRESSION IN-VIVO BY ATHEROGENIC DIETS AND IN-VITRO BY CYTOKINES [J].