Separation of apple procyanidins into different degrees of polymerization by high-speed counter-current chromatography

被引：39

作者：

Shibusawa, Y

Yanagida, A

Isozaki, M

Shindo, H

Ito, Y

机构：

[1] Tokyo Univ Pharm & Life Sci, Sch Pharm, Dept Analyt Chem, Hachioji, Tokyo 1920392, Japan

[2] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 915卷 / 1-2期

关键词：

counter-current chromatography; apples; procyanidins; catechins; tannins;

D O I：

10.1016/S0021-9673(01)00575-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Apple procyanidins were fractionated by high-speed counter-current chromatography in a one-step operation from apple condensed tannins using a type-J multilayer coil planet centrifuge. The separation of procyanidins was performed with a two-phase solvent system composed of methyl acetate-water (1:1) by eluting the upper phase at a flow-rate of 1.0 ml/min. Each fraction was examined by time-of-flight mass spectrometry. Procyanidins were separated according to their degrees of polymerization. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：253 / 257

页数：5

共 20 条

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