Two-dimensional mass spectrometry of biomolecules at the subfemtomole level

被引:37
作者
McLafferty, FW [1 ]
Kelleher, NL [1 ]
Begley, TP [1 ]
Fridriksson, EK [1 ]
Zubarev, RA [1 ]
Horn, DM [1 ]
机构
[1] Cornell Univ, Baker Lab Chem, Ithaca, NY 14853 USA
关键词
D O I
10.1016/S1367-5931(98)80085-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Multiple dimensions of unique molecular structure information can now be obtained from proteins and DNA using mass spectrometry. Less than 10(-16) mol of the active major histocompatibility complex signaling peptide in a mixture of thousands can be identified. For large proteins (>40 kDa), the high resolving power (>10(5)) and 10(-17) mol sensitivity of Fourier-transform mass spectrometry provide exact molecular weight values (+/- 1 or 2 Da) for mixture components, indicating errors or modifications compared with the predicted DNA sequence. Selecting a specific molecular species, its two-dimensional spectrum indicates the part of the molecule that is modified; a three-dimensional spectrum of that fragment further isolates the modification site.
引用
收藏
页码:571 / 578
页数:8
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