Longitudinally profiling neutralizing antibody response to SARS coronavirus with pseudotypes

被引：123

作者：

Temperton, NJ

Chan, PK

Simmons, G

Zambon, MC

Tedder, RS

Takeuchi, Y

Weiss, RA

机构：

[1] UCL, Wohl Vir Ctr, London W1T 4JF, England

[2] UCL, Div Infect & Immun, London W1T 4JF, England

[3] Prince Wales Hosp, Shatin, Hong Kong, Peoples R China

[4] Univ Penn, Sch Med, Philadelphia, PA 19104 USA

[5] Cent Publ Hlth Lab, Hlth Protect Agcy, London NW9 5HT, England

来源：

EMERGING INFECTIOUS DISEASES | 2005年 / 11卷 / 03期

基金：

英国惠康基金;

关键词：

D O I：

10.3201/eid1103.040906

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spike protein (S) is a major target for neutralizing antibodies. Retroviral SARS-CoV S pseudotypes have been constructed and used to develop an in vitro microneutralization assay that is both sensitive and specific for SARS-CoV neutralizing antibodies. Neutralization titers measured by this assay are highly correlated to those measured by an assay using replication-competent SARS-CoV. No cross-neutralization occurred with human sera known to contain antibodies to coronavirus strains OC43 and 229E. The pseudotype assay was used to profile neutralizing antibody responses against SARS-CoV S in sequential serum samples taken from 41 confirmed SARS patients during the 2003 outbreak in Hong Kong and shows long-lasting immunity in most recovered patients. The pseudotype assay does not require handling live SARS virus; it is a useful tool to determine neutralizing titers during natural infection and the preclinical evaluation of candidate vaccines.

引用

页码：411 / 416

页数：6

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