Identification of a calmodulin-regulated autoinhibited Ca2+-ATPase (ACA11) that is localized to vacuole membranes in Arabidopsis

被引：69

作者：

Lee, Sang Min

Kim, Ho Soo

Han, Hay Ju

Moon, Byeong Cheol

Kim, Cha Young

Harper, Jeffery F.

Chung, Woo Sik ^{[1
]}

机构：

[1] Gyeongsang Natl Univ, Plant Mol Biol & Biotechnol Res Ctr, Div Appl Life Sci, BK21 Program, Jinju 660701, South Korea

[2] Gyeongsang Natl Univ, Environm Biotechnol Natl Core Res Ctr, Jinju 660701, South Korea

[3] Korea Inst Oriental Med, Dept Herbal Resources Res, Taejon 305811, South Korea

[4] Univ Nevada, Dept Biochem & Mol Biol, Reno, NV 89557 USA

来源：

FEBS LETTERS | 2007年 / 581卷 / 21期

关键词：

Ca2+-ATPase; calcium; calmodulin; membrane; protein; vacuole; Arabidopsis;

D O I：

10.1016/j.febslet.2007.07.023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In plant cells, the vacuole functions as a major calcium store. Although a calmodulin-regulated Ca2+-ATPase (ACA4) is known to be present 2 in prevacuolar compartments, the presence of an ACA-type Ca2+-ATPase in the mature vacuole of a plant cell has not been verified. Here we provide evidence that ACA11 localizes to the vacuole membrane. ACA11 tagged with GFP was expressed in stable transgenic plants, and visualized in root cells and protoplasts by confocal microscopy. A Ca2+-ATPase function for ACA11 was confirmed by complementation of yeast mutants. A calmodulin binding domain was identified within the first 37 residues of the N-terminal autoinhibitory region. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：3943 / 3949

页数：7

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