Inhibition by gangliosides of Bacillus cereus phospholipase C activity against monolayers, micelles and bilayer vesicles

被引：28

作者：

Daniele, JJ

Maggio, B

Bianco, ID

Goni, FM

Alonso, A

Fidelio, GD

机构：

[1] UNIV NACL CORDOBA, FAC CIENCIAS QUIM,DEPT QUIM BIOL,CIBUIBIC,CONICET, RA-5016 CORDOBA, ARGENTINA

[2] UNIV BASQUE COUNTRY, DEPT BIOQUIM, BILBAO, SPAIN

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1996年 / 239卷 / 01期

关键词：

Bacillus cereus; phospholipase C; gangliosides; interfacial catalysis;

D O I：

10.1111/j.1432-1033.1996.0105u.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of complex glycosphingolipids (gangliosides) on the activity of phospholipase C from Bacillus cereus was studied using lipid monolayers, mixed micelles and small unilamellar vesicles containing phosphatidylcholine as substrate. In all artificial membrane systems assayed, gangliosides exhibit qualitatively similar inhibitory properties. Gangliosides decrease the enzyme activity irrespective of the aggregation structure in which the substrate is offered to B. cereus phospholipase C, and they do not affect the adsorption process of the enzyme. The modulatory effect of gangliosides occurs at the level of the interface, affecting both the maximum rate of catalysis of the enzyme already adsorbed and the availability of the substrate in a suitable organization for enzyme catalysis to take place.

引用

收藏

页码：105 / 110

页数：6

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