Paraxial charge compensator for electron cryomicroscopy

被引：21

作者：

Berriman, John A. ^{[1
]}

Rosenthal, Peter B. ^{[1
]}

机构：

[1] Natl Inst Med Res, MRC, Div Phys Biochem, London NW7 1AA, England

来源：

ULTRAMICROSCOPY | 2012年 / 116卷

基金：

英国医学研究理事会;

关键词：

Cryomicroscopy; Specimen charging; Charge compensation; Transmission electron microscopy; Electrostatic lens; Beam-induced movement; ONE CONDUCTING LAYER; RADIATION-DAMAGE; THIN-FILMS; BIOLOGICAL MACROMOLECULES; CRYOELECTRON MICROSCOPY; STRUCTURAL ORGANIZATION; LOW-TEMPERATURE; SPECIMENS; CONTRAST; IMAGES;

D O I：

10.1016/j.ultramic.2012.03.006

中图分类号：

TH742 [显微镜];

学科分类号：

080401 [精密仪器及机械];

摘要：

We describe a multi-hole condenser aperture for the production of several electron beams in the transmission electron microscope (TEM) making it possible to simultaneously image and irradiate spatially separated regions of a specimen. When the specimen is a thin film of vitreous ice suspended over a holey carbon film, simultaneous irradiation of the adjacent carbon support with the off-axis beam compensates for some of the effects of charging in the image formed by a beam irradiating only the ice. Because the intervening region is not irradiated, charge-neutralization of frozen-hydrated specimens can occur by a through-space mechanism such as the emission of secondary electrons from a grounded carbon support film. We use paraxial charge compensation (PCC) to control the amount of charge build-up on the specimen and observe the effects of charge on images. The multi-hole aperture thus provides a tool for investigating the mechanism of charging and charge mitigation during the imaging of radiation sensitive biological specimens by cryomicroscopy. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：106 / 114

页数：9

共 39 条

[1]

The resolution dependence of optimal exposures in liquid nitrogen temperature electron cryomicroscopy of catalase crystals [J].

Baker, Lindsay A. ;

Smith, Eric A. ;

Bueler, Stephanie A. ;

Rubinstein, John L. .

JOURNAL OF STRUCTURAL BIOLOGY, 2010, 169 (03) :431-437

[2]

Solution structures of DNA-bound gyrase [J].

Baker, Nicole M. ;

Weigand, Steven ;

Maar-Mathias, Sarah ;

Mondragon, Alfonso .

NUCLEIC ACIDS RESEARCH, 2011, 39 (02) :755-766

[3]

Baker T.S., 2006, INT TABLES CRYSTALLO

[4]

Structural organization of Weibel-Palade bodies revealed by cryo-EM of vitrified endothelial cells [J].

Berriman, John A. ;

Li, Sam ;

Hewlett, Lindsay J. ;

Wasilewski, Sebastian ;

Kiskin, Fedir N. ;

Carter, Tom ;

Hannah, Matthew J. ;

Rosenthal, Peter B. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (41) :17407-17412

[5]

ELECTRON CRYOMICROSCOPY OF GRAPHITE IN AMORPHOUS ICE [J].

BOTTCHER, B .

ULTRAMICROSCOPY, 1995, 58 (3-4) :417-424

[6]

Brink J, 1998, J MICROSC-OXFORD, V191, P67

[7]

Evaluation of charging on macromolecules in electron cryomicroscopy [J].

Brink, J ;

Sherman, MB ;

Berriman, J ;

Chiu, W .

ULTRAMICROSCOPY, 1998, 72 (1-2) :41-52

[8]

USE OF SPOT-SCAN PROCEDURE FOR RECORDING LOW-DOSE MICROGRAPHS OF BEAM-SENSITIVE SPECIMENS [J].

BULLOUGH, P ;

HENDERSON, R .

ULTRAMICROSCOPY, 1987, 21 (03) :223-229

[9]

Structural organization of a filamentous influenza A virus [J].

Calder, Lesley J. ;

Wasilewski, Sebastian ;

Berriman, John A. ;

Rosenthal, Peter B. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (23) :10685-10690

[10]

SOME CONSIDERATIONS ON THE ELECTRIC-FIELD INDUCED IN INSULATORS BY ELECTRON-BOMBARDMENT [J].

CAZAUX, J .

JOURNAL OF APPLIED PHYSICS, 1986, 59 (05) :1418-1430

← 1 2 3 4 →