Chemoprevention of tumor metastasis by liposomal β-sitosterol intake

被引:46
作者
Imanaka, Hiromichi [1 ,2 ,3 ]
Koide, Hiroyuki [1 ]
Shimizu, Kosuke [1 ]
Asai, Tomohiro [1 ]
Shimizu, Naomi Kinouchi [1 ]
Ishikado, Atsushi [2 ,3 ]
Makino, Taketoshi [3 ]
Oku, Naoto [1 ]
机构
[1] Univ Shizuoka, Grad Sch Pharmaceut Sci, Dept Med Biochem, Suruga Ku, Shizuoka 4228526, Japan
[2] Univ Shizuoka, Global COE Program, Suruga Ku, Shizuoka 4228526, Japan
[3] Sunstar Inc, R&D Div, Takatsuki, Osaka 5691195, Japan
关键词
liposome; sitosterol; oral delivery; tumor metastasis; chemoprevention;
D O I
10.1248/bpb.31.400
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
To investigate chemopreventive effect of liposomal beta-sitosterol on tumor metastasis, we prepared liposomal beta-sitosterol composed of egg yolk phosphatidylcholine for oral delivery. Although orally administered beta-sitosterol (4 mu mol as beta-sitosterol/mouse) was not absorbed into plasma, the amount of immune response cytokines such as IL-12 and IL-18 was increased in the small intestine after the liposome intake. Moreover, after daily oral administration of the liposome for 7 d, natural killer (NK) cell activity in the mice was increased, suggesting that the immune surveillance activity of mice was enhanced by the lliposomal beta-sitosterol intake. Thus, we examined metastatic potential of B16BL6 melanoma cells, which were intravenously injected into mice after sequential administration of liposomal beta-sitosterol for 7d. The number of metastatic colonies in the lungs was significantly less than that of control group two weeks after the injections of the cells. These results suggest that daily liposomal beta-sitosterol intake prevents tumor metastasis may be due to enhancement of gut immune surveillance systems.
引用
收藏
页码:400 / 404
页数:5
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