A genome-wide screen identifies genes required for centromeric cohesion

被引：214

作者：

Marston, AL ^{[1
]}

Tham, WH ^{[1
]}

Shah, H ^{[1
]}

Amon, A ^{[1
]}

机构：

[1] MIT, Howard Hughes Med Inst, Ctr Canc Res, Cambridge, MA 02139 USA

来源：

SCIENCE | 2004年 / 303卷 / 5662期

关键词：

D O I：

10.1126/science.1094220

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

During meiosis, two chromosome segregation phases follow a single round of DNA replication. We identified factors required to establish this specialized cell cycle by examining meiotic chromosome segregation in a collection of yeast strains lacking all nonessential genes. This analysis revealed Sgo1, Chl4, and Iml3 to be important for retaining centromeric cohesin until the onset of anaphase II. Consistent with this role, Sgo1 localizes to centromeric regions but dissociates at the onset of anaphase II. The screen described here provides a comprehensive analysis of the genes required for the meiotic cell cycle and identifies three factors important for the stepwise loss of sister chromatid cohesion.

引用

页码：1367 / 1370

页数：4

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