Enterococcus faecium QU 50: a novel thermophilic lactic acid bacterium for high-yield L-lactic acid production from xylose

被引：46

作者：

Abdel-Rahman, Mohamed Ali ^{[1
,2
]}

Tashiro, Yukihiro ^{[3
,4
]}

Zendo, Takeshi ^{[1
]}

Sakai, Kenji ^{[3
]}

Sonomoto, Kenji ^{[1
,5
]}

机构：

[1] Kyushu Univ, Lab Microbial Technol, Dept Biosci & Biotechnol, Fac Agr,Grad Sch,Div Syst Bioengn,Higashi Ku, Fukuoka 8128581, Japan

[2] Al Azhar Univ, Dept Bot & Microbiol, Fac Sci, Boys Branch, Cairo 11884, Egypt

[3] Kyushu Univ, Soil Microbiol Lab, Dept Biosci & Biotechnol, Fac Agr,Grad Sch,Div Syst Bioengn,Higashi Ku, Fukuoka 8128581, Japan

[4] Kyushu Univ, Inst Adv Study, Higashi Ku, Fukuoka 8128581, Japan

[5] Kyushu Univ, Lab Funct Food Design, Dept Funct Metab Design, Bioarchitecture Ctr,Higashi Ku, Fukuoka 8128581, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2015年 / 362卷 / 02期

基金：

日本学术振兴会;

关键词：

Lactate fermentation; biomass utilization; biorefinery; pentose; homofermenters; high temperature; REPEATED BATCH FERMENTATION; METABOLISM; SUGARS;

D O I：

10.1093/femsle/fnu030

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

Production of optically pure lactic acid from lignocellulosic material for commercial purposes is hampered by several difficulties, including heterofermentation of pentose sugars and high energy consumption by mesophilic lactic acid bacteria. Here, we report a novel lactic acid bacterium, strain QU 50, that has the potential to produce optically pure l-lactic acid (>= 99.2%) in a homofermentative manner from xylose under thermophilic conditions. Strain QU 50 was isolated from Egyptian fertile soil and identified as Enterococcus faecium QU 50 by analyzing its sugar fermentation pattern and 16S rRNA gene sequence. Enterococcus faecium QU 50 fermented xylose efficiently to produce lactic acid over wide pH (6.0-10.0) and temperature ranges (30-52 degrees C), with a pH of 6.5 and temperature of 50 degrees C being optimal. To our knowledge, this is the first report of homofermentative lactic acid production from xylose by a thermophilic lactic acid bacterium.

引用

页数：7

共 27 条

[1]

Fed-batch fermentation for enhanced lactic acid production from glucose/xylose mixture without carbon catabolite repression [J].