Closing the Gaps in Pediatric Laboratory Reference Intervals: A CALIPER Database of 40 Biochemical Markers in a Healthy and Multiethnic Population of Children

被引：373

作者：

Colantonio, David A. ^{[1
,2
]}

Kyriakopoulou, Lianna ^{[1
,2
]}

Chan, Man Khun ^{[1
]}

Daly, Caitlin H. ^{[1
]}

Brinc, Davor ^{[1
,2
]}

Venner, Allison A. ^{[2
]}

Pasic, Maria D. ^{[2
]}

Armbruster, David ^{[3
]}

Adeli, Khosrow ^{[1
,2
]}

机构：

[1] Hosp Sick Children, Clin Biochem Div, Dept Pediat Lab Med, Toronto, ON M5G 1X8, Canada

[2] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada

[3] Abbott Diagnost, Abbott Pk, IL USA

来源：

CLINICAL CHEMISTRY | 2012年 / 58卷 / 05期

基金：

加拿大健康研究院;

关键词：

BRAIN NATRIURETIC PEPTIDE; CONGENITAL HYPOTHYROIDISM; CHEMISTRY SYSTEM; REFERENCE RANGES; SERUM-ALBUMIN; DYE-BINDING; ADOLESCENTS; AGE; ESTABLISHMENT; ANALYTES;

D O I：

10.1373/clinchem.2011.177741

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

100118 [医学信息学]; 100208 [临床检验诊断学];

摘要：

BACKGROUND: Pediatric healthcare is critically dependent on the availability of accurate and precise laboratory biomarkers of pediatric disease, and on the availability of reference intervals to allow appropriate clinical interpretation. The development and growth of children profoundly influence normal circulating concentrations of biochemical markers and thus the respective reference intervals. There are currently substantial gaps in our knowledge of the influences of age, sex, and ethnicity on reference intervals. We report a comprehensive covariate-stratified reference interval database established from a healthy, nonhospitalized, and multiethnic pediatric population. METHODS: Healthy children and adolescents (n = 2188, newborn to 18 years of age) were recruited from a multiethnic population with informed parental consent and were assessed from completed questionnaires and according to defined exclusion criteria. Whole-blood samples were collected for establishing age- and sex-stratified reference intervals for 40 serum biochemical markers (serum chemistry, enzymes, lipids, proteins) on the Abbott ARCHITECT c8000 analyzer. RESULTS: Reference intervals were generated according to CLSI C28-A3 statistical guidelines. Caucasians, East Asians, and South Asian participants were evaluated with respect to the influence of ethnicity, and statistically significant differences were observed for 7 specific biomarkers. CONCLUSIONS: The establishment of a new comprehensive database of pediatric reference intervals is part of the Canadian Laboratory Initiative in Pediatric Reference Intervals (CALIPER). It should assist laboratorians and pediatricians in interpreting test results more accurately and thereby lead to improved diagnosis of childhood diseases and reduced patient risk. The database will also be of global benefit once reference intervals are validated in transference studies with other analytical platforms and local populations, as recommended by the CLSI. (C) 2012 American Association for Clinical Chemistry

引用

页码：854 / 868

页数：15

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