Sex-specific role of Drosophila melanogaster HP1 in regulating chromatin structure and gene transcription

被引：63

作者：

Liu, LP ^{[1
]}

Ni, JQ ^{[1
]}

Shi, YD ^{[1
]}

Oakeley, EJ ^{[1
]}

Sun, FL ^{[1
]}

机构：

[1] Friedrich Miescher Inst Biomed Res, CH-4058 Basel, Switzerland

来源：

NATURE GENETICS | 2005年 / 37卷 / 12期

关键词：

D O I：

10.1038/ng1662

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Drosophila melanogaster heterochromatin protein 1 (HP1a or HP1)(1) is believed to be involved in active transcription, transcriptional gene silencing and the formation of heterochromatin(2-7). But little is known about the function of HP1 during development. Using a Gal4-induced RNA interference system, we showed that conditional depletion of HP1 in transgenic flies resulted in preferential lethality in male flies. Cytological analysis of mitotic chromosomes showed that HP1 depletion caused sex-biased chromosomal defects, including telomere fusions. The global levels of specific histone modifications, particularly the hallmarks of active chromatin, were preferentially increased in males as well. Expression analysis showed that approximately twice as many genes were specifically regulated by HP1 in males than in females. Furthermore, HP1-regulated genes showed greater enrichment for HP1 binding in males. Taken together, these results indicate that HP1 modulates chromosomal integrity, histone modifications and transcription in a sex-specific manner.

引用

页码：1361 / 1366

页数：6

共 30 条

[1] Activation of transcription through histone H4 acetylation by MOF, an acetyltransferase essential for dosage compensation in Drosophila [J].

Akhtar, A ;

Becker, PB .

MOLECULAR CELL, 2000, 5 (02) :367-375

[2] Regulators of IAP function: coming to grips with the grim reaper [J].

Bergmann, A ;

Yang, AYP ;

Srivastava, M .

CURRENT OPINION IN CELL BIOLOGY, 2003, 15 (06) :717-724

[3]

Bongiorni S, 2001, DEVELOPMENT, V128, P3809

[4] Drosophila Mcm10 interacts with members of the prereplication complex and is required for proper chromosome condensation [J].

Christensen, TW ;

Tye, BK .

MOLECULAR BIOLOGY OF THE CELL, 2003, 14 (06) :2206-2215

[5] Role of Drosophila HP1 in euchromatic gene expression [J].

Cryderman, DE ;

Grade, SK ;

Li, YH ;

Fanti, L ;

Pimpinelli, S ;

Wallrath, LL .

DEVELOPMENTAL DYNAMICS, 2005, 232 (03) :767-774

[6] HP1 modulates the transcription of cell-cycle regulators in Drosophila melanogaster [J].

De Lucia, F ;

Ni, JQ ;

Vaillant, C ;

Sun, FL .

NUCLEIC ACIDS RESEARCH, 2005, 33 (09) :2852-2858

[7] Increased expression of Drosophila Su(var)3-7 triggers Su(var)3-9-dependent heterochromatin formation [J].

Delattre, M ;

Spierer, A ;

Jaquet, Y ;

Spierer, P .

JOURNAL OF CELL SCIENCE, 2004, 117 (25) :6239-6247

[8] Su(var) genes regulate the balance between euchromatin and heterochromatin in Drosophila [J].

Ebert, A ;

Schotta, G ;

Lein, S ;

Kubicek, S ;

Krauss, V ;

Jenuwein, T ;

Reuter, G .

GENES & DEVELOPMENT, 2004, 18 (23) :2973-2983

[9] MUTATION IN A HETEROCHROMATIN-SPECIFIC CHROMOSOMAL PROTEIN IS ASSOCIATED WITH SUPPRESSION OF POSITION-EFFECT VARIEGATION IN DROSOPHILA-MELANOGASTER [J].

EISSENBERG, JC ;

JAMES, TC ;

FOSTERHARTNETT, DM ;

HARTNETT, T ;

NGAN, V ;

ELGIN, SCR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (24) :9923-9927

[10] The Heterochromatin protein 1 prevents telomere fusions in Drosophila [J].

Fanti, L ;

Giovinazzo, G ;

Berloco, M ;

Pimpinelli, S .

MOLECULAR CELL, 1998, 2 (05) :527-538

← 1 2 3 →