Protocol for the MPTP mouse model of Parkinson's disease

被引：833

作者：

Jackson-Lewis, Vernice ^{[1
]}

Przedborski, Serge ^{[1
,2
]}

机构：

[1] Columbia Univ, Dept Neurol, New York, NY 10032 USA

[2] Columbia Univ, Dept Pathol & Cell Biol, New York, NY 10032 USA

来源：

NATURE PROTOCOLS | 2007年 / 2卷 / 01期

关键词：

D O I：

10.1038/nprot.2006.342

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This protocol describes our method of producing a reliable mouse model of Parkinson's disease (PD) using the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). We discuss the particulars of the model, provide key references and outline what investigators need to know to develop the MPTP mouse model of PD safely and successfully. Completion of this protocol depends on the regimen of MPTP used and on the actual planned studies, which often range from 7 to 30 d. This protocol calls for implementation of safety measures and for the acquisition of several pieces of equipment, which are a one-time investment worth making if one elects to use this model on a regular basis.

引用

页码：141 / 151

页数：11

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