Identification of TogMNAB, an ABC transporter which mediates the uptake of pectic oligomers in Erwinia chrysanthemi 3937

被引:40
作者
Hugouvieux-Cotte-Pattat, N [1 ]
Blot, N [1 ]
Reverchon, S [1 ]
机构
[1] Composante INSA, Unite Microbiol & Genet, UMR UCB INSA, CNRS 5122, F-69621 Villeurbanne, France
关键词
D O I
10.1046/j.1365-2958.2001.02564.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The bacterium Erwinia chrysanthemi, which causes soft rot disease on various plants, is able to use pectin as a carbon source for growth. Knowledge of the critical step in pectin catabolism which allows the entry of pectic oligomers into the cells is scarce. We report here the first example of a transport system involved in the uptake of pectic oligomers. The TogMNAB transporter of E. chrysanthemi is a member of the ATP-binding cassette (ABC) superfamily. TogM and TogN are homologous to the inner membrane components, TogA exhibits the signature of ABC ATPases and TogB shows similarity with periplasmic ligand-binding proteins. The TogMNAB transporter is a new member of the carbohydrate uptake transporter-1 family (CUT1, TC no. 3.1.1), which is specialized in the transport of complex sugars. The four genes, togM, togN, togA and togB, are apparently co-transcribed in a large operon which also includes the pectate lyase gene pelW. The transcription of the tog operon is induced in the presence of pectic derivatives and is affected by catabolite repression. It is controlled by the KdgR repressor and the CRP activator. The TogMNAB system is able to provide Escherichia coli with the ability to transport oligogalacturonides. In E. chrysanthemi, the TogMNAB system seems to play a major role in switching on the induction of pectin catabolism. TogB also acts as a specific receptor for chemotaxis towards oligogalacturonides. The decreased capacity of maceration of a togM mutant indicates the importance of transport and/or attraction of oligogalacturonides for E. chrysanthemi pathogenicity.
引用
收藏
页码:1113 / 1123
页数:11
相关论文
共 46 条
[11]   ENVIRONMENTAL-CONDITIONS AFFECT TRANSCRIPTION OF THE PECTINASE GENES OF ERWINIA-CHRYSANTHEMI 3937 [J].
HUGOUVIEUXCOTTEPATTAT, N ;
DOMINGUEZ, H ;
ROBERTBAUDOUY, J .
JOURNAL OF BACTERIOLOGY, 1992, 174 (23) :7807-7818
[12]   Regulation of pectinolysis in Erwinia chrysanthemi [J].
HugouvieuxCottePattat, N ;
Condemine, G ;
Nasser, W ;
Reverchon, S .
ANNUAL REVIEW OF MICROBIOLOGY, 1996, 50 :213-257
[13]   ALDOHEXURONATE TRANSPORT-SYSTEM IN ERWINIA-CAROTOVORA [J].
HUGOUVIEUXCOTTEPATTAT, N ;
QUESNEAU, Y ;
ROBERTBAUDOUY, J .
JOURNAL OF BACTERIOLOGY, 1983, 154 (02) :663-668
[14]   HEXURONATE CATABOLISM IN ERWINIA-CHRYSANTHEMI [J].
HUGOUVIEUXCOTTEPATTAT, N ;
ROBERTBAUDOUY, J .
JOURNAL OF BACTERIOLOGY, 1987, 169 (03) :1223-1231
[15]   The Escherichia coli ATP-binding cassette (ABC) proteins [J].
Linton, KJ ;
Higgins, CF .
MOLECULAR MICROBIOLOGY, 1998, 28 (01) :5-13
[16]   CHARACTERIZATION OF THE PEIL GENE ENCODING A NOVEL PECTATE LYASE OF ERWINIA-CHRYSANTHEMI-3937 [J].
LOJKOWSKA, E ;
MASCLAUX, C ;
BOCCARA, M ;
ROBERTBAUDOUY, J ;
HUGOUVIEUXCOTTEPATTAT, N .
MOLECULAR MICROBIOLOGY, 1995, 16 (06) :1183-1195
[17]   SPECIFIC INTERACTIONS OF ERWINIA-CHRYSANTHEMI KDGR REPRESSOR WITH DIFFERENT OPERATORS OF GENES INVOLVED IN PECTINOLYSIS [J].
NASSER, W ;
REVERCHON, S ;
CONDEMINE, G ;
ROBERTBAUDOUY, J .
JOURNAL OF MOLECULAR BIOLOGY, 1994, 236 (02) :427-440
[18]   Analysis of three clustered polygalacturonase genes in Erwinia chrysanthemi 3937 revealed an anti-repressor function for the PecS regulator [J].
Nasser, W ;
Shevchik, VE ;
Hugouvieux-Cotte-Pattat, N .
MOLECULAR MICROBIOLOGY, 1999, 34 (04) :641-650
[19]   Antagonistic effect of CRP and KdgR in the transcription control of the Erwinia chrysanthemi pectinolysis genes [J].
Nasser, W ;
Robert-Baudouy, J ;
Reverchon, S .
MOLECULAR MICROBIOLOGY, 1997, 26 (05) :1071-1082
[20]   NUCLEOTIDE-SEQUENCE OF THE UGP GENES OF ESCHERICHIA-COLI K-12 - HOMOLOGY TO THE MALTOSE SYSTEM [J].
OVERDUIN, P ;
BOOS, W ;
TOMMASSEN, J .
MOLECULAR MICROBIOLOGY, 1988, 2 (06) :767-775