Comparative plant genomics resources at PlantGDB

被引:61
作者
Dong, QF
Lawrence, CJ
Schlueter, SD
Wilkerson, MD
Kurtz, S
Lushbough, C
Brendel, V [1 ]
机构
[1] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA 50011 USA
[2] Iowa State Univ, Dept Stat, Ames, IA 50011 USA
[3] Univ Hamburg, Zentrum Bioinformat, D-20146 Hamburg, Germany
[4] Univ S Dakota, Dept Comp Sci, Vermillion, SD 57069 USA
关键词
D O I
10.1104/pp.104.059212
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
PlantGDB (http://www.plantgdb.org/)is a database of plant molecular sequences. Expressed sequence tag (EST) sequences are assembled into contigs that represent tentative unique genes. EST contigs are functionally annotated with information derived from known protein sequences that are highly similar to the putative translation products. Tentative Gene Ontology terms are assigned to match those of the similar sequences identified. Genome survey sequences are assembled similarly. The resulting genome survey sequence contigs are matched to ESTs and conserved protein homologs to identify putative full-length open reading frame-containing genes, which are subsequently provisionally classified according to established gene family designations. For Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa), the exon-intron boundaries for gene structures are annotated by spliced alignment of ESTs and full-length cDNAs to their respective complete genome sequences. Unique genome browsers have been developed to present all available EST and cDNA evidence for current transcript models (for Arabidopsis, see the AtGDB site at http://www.plantgdb.org/AtGDB/; for rice, see the OsGDB site at http://www.plantgdb.org/ OsGDB/). In addition, a number of bioinformatic tools have been integrated at PlantGDB that enable researchers to carry out sequence analyses on-site using both their own data and data residing within the database.
引用
收藏
页码:610 / 618
页数:9
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