The association of peroxisomes with the developing cell plate in dividing onion root cells depends on actin microfilaments and myosin

被引:34
作者
Collings, DA
Harper, JDI
Vaughn, KC
机构
[1] Australian Natl Univ, Res Sch Biol Sci, Plant Cell Biol Grp, Canberra, ACT 2601, Australia
[2] Univ Sydney, Sch Biol Sci, Sydney, NSW 2006, Australia
[3] Charles Sturt Univ, Sch Agr, Farrer Ctr, Wagga Wagga, NSW 2678, Australia
[4] ARS, So Weed Sci Res Unit, USDA, Stoneville, MS 38776 USA
基金
澳大利亚研究理事会;
关键词
actin microfilaments; Allium; microtubule; cell plate; peroxisome; phragmoplast;
D O I
10.1007/s00425-003-1096-2
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We have investigated changes in the distribution of peroxisomes through the cell cycle in onion (Allium cepa L.) root meristem cells with immunofluorescence and electron microscopy, and in leek (Allium porrum L.) epidermal cells with immunofluorescence and peroxisomal-targeted green fluorescent protein. During interphase and mitosis, peroxisomes distribute randomly throughout the cytoplasm, but beginning late in anaphase, they accumulate at the division plane. Initially, peroxisomes occur within the microtubule phragmoplast in two zones on either side of the developing cell plate. However, as the phragmoplast expands outwards to form an annulus, peroxisomes redistribute into a ring immediately inside the location of the microtubules. Peroxisome aggregation depends on actin microfilaments and myosin. Peroxisomes first accumulate in the division plane prior to the formation of the microtubule phragmoplast, and throughout cytokinesis, always co-localise with microfilaments. Microfilament-disrupting drugs (cytochalasin and latrunculin), and a putative inhibitor of myosin (2,3-butanedione monoxime), inhibit aggregation. We propose that aggregated peroxisomes function in the formation of the cell plate, either by regulating hydrogen peroxide production within the developing cell plate, or by their involvement in recycling of excess membranes from secretory vesicles via the beta-oxidation pathway. Differences in aggregation, a phenomenon which occurs in onion, some other monocots and to a lesser extent in tobacco BY-2 suspension cells, but which is not obvious in the roots of Arabidopsis thaliana (L.) Heynh., may reflect differences within the primary cell walls of these plants.
引用
收藏
页码:204 / 216
页数:13
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