The structural integrity of the endoplasmic reticulum, and its possible regulation by inositol 1,3,4,5-tetrakisphosphate

被引：13

作者：

Brough, D ^{[1
]}

Sim, Y ^{[1
]}

Thorn, P ^{[1
]}

Irvine, RF ^{[1
]}

机构：

[1] Univ Cambridge, Dept Pharmacol, Cambridge CB2 1PD, England

来源：

CELL CALCIUM | 2005年 / 38卷 / 02期

基金：

英国惠康基金;

关键词：

endoplasmic reticulum; calcium; inositol tetrakisphosphate; fragmentation;

D O I：

10.1016/j.ceca.2005.05.002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 [细胞生物学]; 090102 [作物遗传育种];

摘要：

The endoplasmic reticulum (ER) is a dynamic organelle thought to consist of a single interconnected network of membranes. Using fluorescence recovery after photobleaching (FRAP) of HEK-293 cells dually transfected with soluble fluorescent proteins targeted to the ER (GFP) and mitochondria (DsRed), we have confirmed this continuity, which contrasts that of the mitochondria, which behave as a population of discrete organelles. The degree of ER integrity (interconnected versus fragmented) has been suggested to be regulated in some cells by inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P-4). In HEK-293 and freshly isolated murine lacrimal acinar cells, we manipulated ER structure by disrupting cellular Ca2+ homeostasis with the Ca2+ ionophore ionomycin, and by permeabilisation of the plasma membrane, protocols known to cause ER fragmentation. However, we were subsequently unable to detect by FRAP any significant effect of Ins(1,3,4,5)P-4 on ER integrity.(c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：153 / 159

页数：7

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