Reishi immuno-modulation protein induces interleukin-2 expression via protein kinase-dependent signaling pathways within human T cells

被引：45

作者：

Hsu, Hsien-Yeh ^{[2
,3
,4
]}

Hua, Kuo-Feng ^{[2
,3
]}

Wu, Wei-Chi ^{[2
]}

Hsu, Jason ^{[5
]}

Weng, Shih-Ting ^{[2
]}

Lin, Tsai-Leng ^{[1
]}

Liu, Chun-Yi ^{[2
]}

Hseu, Ruey-Shyang ^{[1
]}

Huang, Ching-Tsan ^{[1
]}

机构：

[1] Natl Taiwan Univ, Inst Microbiol & Biochem, Dept Biochem Sci & Technol, Taipei 10764, Taiwan

[2] Natl Yang Ming Univ, Lab Sci Med, Taipei 112, Taiwan

[3] Natl Yang Ming Univ, Inst Biophoton Engn, Taipei 112, Taiwan

[4] Taipei City Hosp, Dept Educ & Res, Taipei, Taiwan

[5] Univ Michigan, Stephen M Ross Sch Business, Ann Arbor, MI 48109 USA

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 2008年 / 215卷 / 01期

关键词：

D O I：

10.1002/jcp.21144

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Ganoderma lucidum, a medicinal fungus is thought to possess and enhance a variety of human immune functions. An immuno-modulatory protein, Ling Zhi-8 (LZ-8) isolated from G. lucidum exhibited potent mitogenic effects upon human peripheral blood lymphocytes (PBL). However, LZ-8-mediated signal transduction in the regulation of interleukin-2 (IL-2) gene expression within human T cells is largely unknown. Here we cloned the LZ-8 gene of G. lucidum, and expressed the recombinant LZ-8 protein (rLZ-8) by means of a yeast Pichia pastoris protein expression system. We found that rLZ-8 induces IL-2 gene expression via the Src-family protein tyrosine kinase (PTK), via reactive oxygen species (ROS), and differential protein kinase-dependent pathways within human primary T cells and cultured Jurkat T cells. In essence, we have established the nature of the rLZ-8-mediated signal-transduction pathways, such as PTK/protein kinase C (PKC)/ROS, PTK/PLC/PKCalpha/ERK 1/2, and PTK/PLC/PKCalpha/p38 pathways in the regulation of IL-2 gene expression within human T cells. Our current results of analyzing rLZ-8-mediated signal transduction in T cells might provide a potential application for rLZ-8 as a pharmacological immune-modulating agent.

引用

页码：15 / 26

页数：12

共 43 条

[1] A simple method for extraction of fungal genomic DNA [J].

Al-Samarrai, TH ;

Schmid, J .

LETTERS IN APPLIED MICROBIOLOGY, 2000, 30 (01) :53-56

[2] INOSITOL TRISPHOSPHATE AND CALCIUM SIGNALING [J].

BERRIDGE, MJ .

NATURE, 1993, 361 (6410) :315-325

[3] Studies on the immuno-modulating and anti-tumor activities of Ganoderma lucidum (Reishi) polysaccharides [J].

Chen, HS ;

Tsai, YF ;

Lin, S ;

Lin, CC ;

Khoo, KH ;

Lin, CH ;

Wong, CH .

BIOORGANIC & MEDICINAL CHEMISTRY, 2004, 12 (21) :5595-5601

[4] Polysaccharides of Ganoderma lucidum alter cell immunophenotypic expression and enhance CD56+ NK-cell cytotoxicity in cord blood [J].

Chien, CA ;

Cheng, JL ;

Chang, WT ;

Tien, MH ;

Tsao, CM ;

Chang, YH ;

Chang, HY ;

Hsieh, JF ;

Wong, CH ;

Chen, ST .

BIOORGANIC & MEDICINAL CHEMISTRY, 2004, 12 (21) :5603-5609

[5] CALCIUM SIGNALING [J].

CLAPHAM, DE .

CELL, 1995, 80 (02) :259-268

[6] JNK is required for effector T-cell function but not for T-cell activation [J].

Dong, C ;

Yang, DD ;

Tournier, C ;

Whitmarsh, AJ ;

Xu, J ;

Davis, RJ ;

Flavell, RA .

NATURE, 2000, 405 (6782) :91-94

[7] CALCINEURIN ACTS IN SYNERGY WITH PMA TO INACTIVATE I-KAPPA-B/MAD3, AN INHIBITOR OF NF-KAPPA-B [J].

FRANTZ, B ;

NORDBY, EC ;

BREN, G ;

STEFFAN, N ;

PAYA, CV ;

KINCAID, RL ;

TOCCI, MJ ;

OKEEFE, SJ ;

ONEILL, EA .

EMBO JOURNAL, 1994, 13 (04) :861-870

[8] Interleukin 2 signaling is required for CD4+ regulatory T cell function [J].

Furtado, GC ;

de Lafaille, MAC ;

Kutchukhidze, N ;

Lafaille, JJ .

JOURNAL OF EXPERIMENTAL MEDICINE, 2002, 196 (06) :851-857

[9] LING ZHI-8 - A NOVEL T-CELL MITOGEN INDUCES CYTOKINE PRODUCTION AND UP-REGULATION OF ICAM-1 EXPRESSION [J].

HAAKFRENDSCHO, M ;

KINO, K ;

SONE, T ;

JARDIEU, P .

CELLULAR IMMUNOLOGY, 1993, 150 (01) :101-113

[10] CD45: A critical regulator of signaling thresholds in immune cells [J].

Hermiston, ML ;

Xu, Z ;

Weiss, A .

ANNUAL REVIEW OF IMMUNOLOGY, 2003, 21 :107-137

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