Reishi immuno-modulation protein induces interleukin-2 expression via protein kinase-dependent signaling pathways within human T cells

被引：45

作者：

Hsu, Hsien-Yeh ^{[2
,3
,4
]}

Hua, Kuo-Feng ^{[2
,3
]}

Wu, Wei-Chi ^{[2
]}

Hsu, Jason ^{[5
]}

Weng, Shih-Ting ^{[2
]}

Lin, Tsai-Leng ^{[1
]}

Liu, Chun-Yi ^{[2
]}

Hseu, Ruey-Shyang ^{[1
]}

Huang, Ching-Tsan ^{[1
]}

机构：

[1] Natl Taiwan Univ, Inst Microbiol & Biochem, Dept Biochem Sci & Technol, Taipei 10764, Taiwan

[2] Natl Yang Ming Univ, Lab Sci Med, Taipei 112, Taiwan

[3] Natl Yang Ming Univ, Inst Biophoton Engn, Taipei 112, Taiwan

[4] Taipei City Hosp, Dept Educ & Res, Taipei, Taiwan

[5] Univ Michigan, Stephen M Ross Sch Business, Ann Arbor, MI 48109 USA

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 2008年 / 215卷 / 01期

关键词：

D O I：

10.1002/jcp.21144

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Ganoderma lucidum, a medicinal fungus is thought to possess and enhance a variety of human immune functions. An immuno-modulatory protein, Ling Zhi-8 (LZ-8) isolated from G. lucidum exhibited potent mitogenic effects upon human peripheral blood lymphocytes (PBL). However, LZ-8-mediated signal transduction in the regulation of interleukin-2 (IL-2) gene expression within human T cells is largely unknown. Here we cloned the LZ-8 gene of G. lucidum, and expressed the recombinant LZ-8 protein (rLZ-8) by means of a yeast Pichia pastoris protein expression system. We found that rLZ-8 induces IL-2 gene expression via the Src-family protein tyrosine kinase (PTK), via reactive oxygen species (ROS), and differential protein kinase-dependent pathways within human primary T cells and cultured Jurkat T cells. In essence, we have established the nature of the rLZ-8-mediated signal-transduction pathways, such as PTK/protein kinase C (PKC)/ROS, PTK/PLC/PKCalpha/ERK 1/2, and PTK/PLC/PKCalpha/p38 pathways in the regulation of IL-2 gene expression within human T cells. Our current results of analyzing rLZ-8-mediated signal transduction in T cells might provide a potential application for rLZ-8 as a pharmacological immune-modulating agent.

引用

页码：15 / 26

页数：12

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