The C-terminal domain revealed in the structure of RNA polymerase II

被引：31

作者：

Meredith, GD

Chang, WH

Li, Y

Bushnell, DA

Darst, SA

Kornberg, RD

机构：

[1] STANFORD UNIV,SCH MED,DEPT BIOL STRUCT,STANFORD,CA 94305

[2] ROCKEFELLER UNIV,NEW YORK,NY 10021

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1996年 / 258卷 / 03期

关键词：

RNA polymerase II; CTD; structure; electron crystallography; transcription;

D O I：

10.1006/jmbi.1996.0258

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The location of the CTD in the structure of RNA polymerase II has been determined by electron crystallography at 16 Angstrom resolution. Difference maps between wild-type enzyme and that lacking the CTD, or with an antibody fragment bound in place of the CTD, disclose the site of attachment of the CTD to the polymerase. Appropriate display of the polymerase structure reveals the CTD as an element projecting from this site of attachment into solution. A low relative density and large volume of this element identify the CTD as a conformationally mobile region. (C) 1996 Academic Press Limited

引用

收藏

页码：413 / 419

页数：7

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