A NOVEL METHOD FOR TRANSFER OF 2-DIMENSIONAL CRYSTALS FROM THE AIR-WATER-INTERFACE TO SPECIMEN GRIDS - EM-SAMPLE PREPARATION LIPID-LAYER CRYSTALLIZATION

被引：20

作者：

ASTURIAS, FJ

KORNBERG, RD

机构：

[1] Department of Cell Biology, Stanford University School of Medicine, Stanford

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 1995年 / 114卷 / 01期

关键词：

D O I：

10.1006/jsbi.1995.1005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transfer of two-dimensional (2-D) crystals formed on lipid layers by suspension from a wire loop is described. This method gives better recovery and better preservation of 2-D crystals than attained in the past. The method has been applied to crystals of yeast RNA polymerase II to enable their analysis in the frozen hydrated state. (C) 1995 Academic Press, Inc.

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页码：60 / 66

页数：7

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