Neurotrophin-induced transport of a β-actin mRNP complex increases β-actin levels and stimulates growth cone motility

被引：316

作者：

Zhang, HL

Eom, T

Oleynikov, Y

Shenoy, SM

Liebelt, DA

Dictenberg, JB

Singer, RH

Bassell, GJ

机构：

[1] Yeshiva Univ Albert Einstein Coll Med, Rose F Kennedy Ctr Mental Retardat, Dept Neurosci, Bronx, NY 10461 USA

[2] Yeshiva Univ Albert Einstein Coll Med, Dept Anat & Struct Biol, Bronx, NY 10461 USA

来源：

NEURON | 2001年 / 31卷 / 02期

关键词：

D O I：

10.1016/S0896-6273(01)00357-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Neurotrophin regulation of actin-dependent changes in growth cone motility may depend on the signaling of beta -actin mRNA transport. Formation of an RNP complex between the beta -actin mRNA zipcode sequence and Zip-code Binding Protein 1 (ZBP1) was required for its localization to growth cones. Antisense oligonucleotides to the zipcode inhibited formation of this RNP complex in vitro and the neurotrophin-induced localization of beta -actin mRNA and ZBP1 granules. Live cell imaging of neurons transfected with EGFP-ZBP1 revealed fast, bidirectional movements of granules in neurites that were inhibited by antisense treatment, as visualized by FRAP analysis. NT-3 stimulation of P-actin protein localization was dependent on the 3'UTR and inhibited by antisense treatment. Growth cones exhibited impaired motility in the presense of antisense. These results suggest a novel mechanism to influence growth cone dynamics involving the regulated transport of mRNA.

引用

页码：261 / 275

页数：15

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