Evaluation of the concept of heterology in a monoclonal antibody-based ELISA utilizing direct hapten linkage to polystyrene microtiter plates

被引：30

作者：

Holthues, H ^{[1
]}

Pfeifer-Fukumura, U ^{[1
]}

Sound, I ^{[1
]}

Baumann, W ^{[1
]}

机构：

[1] Univ Mainz, Inst Phys Chem, D-55128 Mainz, Germany

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2005年 / 304卷 / 1-2期

关键词：

hapten; heterology; immunoassay; triazines;

D O I：

10.1016/j.jim.2005.06.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of new heterologous haptens has been synthesized and used as coating haptens in an antigen-immobilized immunoassay with a monoclonal antibody against atrazine. Coating was achieved by covalently linking the different haptens to a glutaraldehyde network directly bound to the polystyrene surface of a standard 96-well microtiter plate. With the assay designed in the antigen-immobilized format with direct chemical linkage of the hapten to the solid polystyrene surface well-defined hapten densities were achieved in all experiments. The results of different experiments with different coating haptens were comparable. Using different heterologous haptens it appears that the concept of heterology is applicable in this case and can be used to enhance the sensitivity of an immunoassay in the coating-hapten format. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：68 / 77

页数：10

共 29 条

[21] ENVIRONMENTAL CHEMISTRY - THE IMMUNOASSAY OPTION [J].