Structural elements required for replication and incompatibility of the Rhizobium etli symbiotic plasmid

被引：56

作者：

Ramírez-Romero, MA ^{[1
]}

Soberón, N ^{[1
]}

Pérez-Oseguera, A ^{[1
]}

Téllez-Sosa, J ^{[1
]}

Cevallos, MA ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Ctr Invest Sobre Fijac Nitrogeno, Programa Evoluc Mol, Cuernavaca 62191, Morelos, Mexico

来源：

JOURNAL OF BACTERIOLOGY | 2000年 / 182卷 / 11期

关键词：

D O I：

10.1128/JB.182.11.3117-3124.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The symbiotic plasmid of Rhizobium etli CE3 belongs to the RepABC family of plasmid replicons. This family is characterized by the presence of three conserved genes, repA, repB, and repC, encoded by the same DNA strand. A long intergenic sequence (igs) between repB and repC is also conserved in all members of the plasmid family. In this paper we demonstrate that (i) the repABC genes are organized in an operon; (ii) the RepC product is essential for replication; (iii) RepA and RepB products participate in plasmid segregation and in the regulation of plasmid copy number; (iv) there are two cis-acting incompatibility regions, one located in the igs (inc alpha) and the other downstream of repC (inc beta) (the former is essential for replication); and (v) RepA is a trans-acting incompatibility factor. We suggest that inca is a cis-acting site required for plasmid partitioning and that the origin of replication lies within inc beta.

引用

页码：3117 / 3124

页数：8

共 44 条

[1] PARTITION OF UNIT-COPY MINIPLASMIDS TO DAUGHTER CELLS .3. THE DNA-SEQUENCE AND FUNCTIONAL-ORGANIZATION OF THE P1-PARTITION REGION [J].