In situ monitoring of DNA:: the plant nuclear envelope allows passage of short DNA fragments

被引：4

作者：

Gisel, A

Rothen, B

Iglesias, VA

Potrykus, I

Sautter, C

机构：

[1] ETH Zurich, Inst Plant Sci, CH-8092 Zurich, Switzerland

[2] ETH Honggerberg, Inst Cell Biol, CH-8093 Zurich, Switzerland

来源：

PLANT JOURNAL | 1998年 / 16卷 / 05期

关键词：

D O I：

10.1046/j.1365-313x.1998.00323.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We monitored the cellular localization of fluorescently labeled foreign DNA in living plant cells. After physical delivery of labeled DNA fragments to the cytoplasm, short fragments up to 1.5 kb in length were found equally distributed between the cytoplasm and nucleus after 60 min. In contrast, 2.5 kb DNA fragments did not appear inside the nucleus. Thus, foreign DNA can enter plant nuclei through the intact nuclear envelope, but the efficiency of this process declines with increasing size of fragment.

引用

页码：621 / 626

页数：6

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