EFFECT OF DNA FRAGMENT SIZE ON TRANSFORMATION FREQUENCIES IN TOBACCO (NICOTIANA-TABACUM) AND MAIZE (ZEA-MAYS)

被引：5

作者：

FLEMING, GH ^{[1
]}

KRAMER, CM ^{[1
]}

LE, T ^{[1
]}

SHILLITO, RD ^{[1
]}

机构：

[1] CIBA GEIGY CORP,AGR BIOTECHNOL RES UNIT,RES TRIANGLE PK,NC 27709

来源：

PLANT SCIENCE | 1995年 / 110卷 / 02期

关键词：

PROTOPLASTS; NICOTIANA TABACUM; ZEA MAYS; STABLE TRANSFORMATION; TRANSIENT EXPRESSION; DNA FRAGMENT SIZE;

D O I：

10.1016/0168-9452(95)04196-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

During eukaryotic cell transformation, the transforming DNA must enter the host cell, traverse the cytoplasm and enter the nucleus before becoming stably integrated into the genome. The limiting step for plant protoplast transformation may lie at the cell membrane, the nuclear membrane, or at the integration step. We, show here that the size of the DNA fragment containing the selectable marker used to monitor transformation can directly affect the efficiency of stable transformation. In both tobacco and maize protoplasts, the smallest DNA fragments gave the highest stable transformation frequencies.

引用

页码：187 / 192

页数：6

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