Determination of ethinylestradiol and gestodene in oral contraceptives by micellar electrokinetic chromatography

被引：9

作者：

Berzas, JJ ^{[1
]}

Rodríguez, J ^{[1
]}

Castañeda, G ^{[1
]}

Pinilla, MJ ^{[1
]}

机构：

[1] Univ Castilla La Mancha, Fac Ciencias, Dept Quim Analit & Tecnol Alimentos, E-13071 Ciudad Real, Spain

来源：

CHROMATOGRAPHIA | 1999年 / 49卷 / 1-2期

关键词：

column liquid chromatography; micellar electrokinetic chromatography (MEKC); oral contraceptives; ethinylestradiol; gestodene;

D O I：

10.1007/BF02467189

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A micellar electrokinetic chromatographic method is presented which permits quantification of ethinylestradiol and gestodene in pharmaceutical products. Separation was carried out at 25 degrees C and 25 KV, using a 20 mM berate buffer (pH 9.2), 15 mM sodium dodecylsulfate in 30% acetonitrile-water (v/v). Under these conditions analyses were carried out in 7 min. Four different oral contraceptives were analysed and the results compared favourably with those of a reference liquid chromatographic method.

引用

页码：65 / 70

页数：6

共 19 条

[1] APPROACHES TO OPTIMIZATION OF PRECISION IN CAPILLARY ELECTROPHORESIS [J].

ALTRIA, KD ;

FABRE, H .

CHROMATOGRAPHIA, 1995, 40 (5-6) :313-320

[2] VALIDATION OF A CAPILLARY ELECTROPHORESIS METHOD FOR THE DETERMINATION OF A QUINOLONE ANTIBIOTIC AND ITS RELATED IMPURITIES [J].

ALTRIA, KD ;

CHANTER, YL .

JOURNAL OF CHROMATOGRAPHY A, 1993, 652 (02) :459-463

[3] ESSENTIAL PEAK AREA NORMALIZATION FOR QUANTITATIVE IMPURITY CONTENT DETERMINATION BY CAPILLARY ELECTROPHORESIS [J].

ALTRIA, KD .

CHROMATOGRAPHIA, 1993, 35 (3-4) :177-182

[4] QUANTITATIVE ASPECTS OF THE APPLICATION OF CAPILLARY ELECTROPHORESIS TO THE ANALYSIS OF PHARMACEUTICALS AND DRUG-RELATED IMPURITIES [J].

ALTRIA, KD .

JOURNAL OF CHROMATOGRAPHY, 1993, 646 (02) :245-257

[5] Quantitation of vaginally administered nonoxynol-9 in premenopausal women [J].

BarditchCrovo, P ;

Witter, F ;

Hamzeh, F ;

McPherson, J ;

Stratton, P ;

Alexander, NJ ;

Trapnell, CB .

CONTRACEPTION, 1997, 55 (04) :261-263

[6]

Berzas JJ, 1997, ANAL SCI, V13, P1029

[7]

Berzas Nevado J. J., 1997, Analytical Letters, V30, P2221

[8]

CASTANEDA E, 1996, CHROMATOGRAPHIA, V42, P159

[9] PROBLEMS OF QUANTITATIVE INJECTION IN CAPILLARY ZONE ELECTROPHORESIS [J].

DOSE, EV ;

GUIOCHON, G .

ANALYTICAL CHEMISTRY, 1992, 64 (02) :123-128

[10] RAPID, SENSITIVE COLORIMETRIC METHOD FOR DETERMINATION OF ETHINYL ESTRADIOL [J].

ELDAWY, MA ;

TAWFIK, AS ;

ELSHABOURI, SR .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1975, 64 (07) :1221-1223

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