Chitosan beads as molecularly imprinted polymer matrix for selective separation of proteins

被引：158

作者：

Guo, TY ^{[1
]}

Xia, YQ ^{[1
]}

Wang, J ^{[1
]}

Song, MD ^{[1
]}

Zhang, BH ^{[1
]}

机构：

[1] Nankai Univ, Coll Chem, State Key Lab Funct Polymer Mat Adsorpt & Seperat, Inst Polymer Chem, Tianjin 300071, Peoples R China

来源：

BIOMATERIALS | 2005年 / 26卷 / 28期

基金：

中国国家自然科学基金;

关键词：

chitosan beads; polyacrylamide; protein separation; molecular imprinting;

D O I：

10.1016/j.biomaterials.2005.02.017

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Two kinds of molecularly imprinted polymers were prepared using hemoglobin as the imprinting molecule, acrylamide as the functional monomer, chitosan beads and maleic anhydride-modified chitosan beads as matrixes, respectively. Static adsorbing experimental results showed that an equal class of adsorption was formed in the imprinted polymers and the adsorption equilibrium constant and the maximum adsorption capacity were evaluated. Chromatographic characteristics showed that the column bedded with the hemoglobin imprinted beads could separate hemoglobin and bovine serum albumin effectively from their mixture, which indicates that the imprinted beads have very higher selectivity for hemoglobin than the non-imprinted with the same chemical composition. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：5737 / 5745

页数：9

共 26 条

[1] IMPRINTING OF AMINO-ACID DERIVATIVES IN MACROPOROUS POLYMERS [J].