Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of Staphylococcus aureus in food

被引：32

作者：

Yang, Hong ^{[1
]}

Ma, Xiaoyan ^{[1
]}

Zhang, Xianzhou ^{[1
]}

Wang, Yu ^{[1
]}

Zhang, Wei ^{[1
]}

机构：

[1] Agr Univ HeBei, Coll Food Sci & Technol, Baoding 071000, Peoples R China

来源：

EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2011年 / 232卷 / 05期

关键词：

Loop-mediated isothermal amplification; LAMP; Detection; Staphylococcus aureus; Food; ENTEROTOXINS;

D O I：

10.1007/s00217-011-1442-8

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Staphylococcus aureus in food was developed and evaluated. Heat-stable nuclease (nuc) gene was the target sequence amplified by LAMP. LAMP includes primer design, amplification at 64 A degrees C, macroscopic observation, and estimation of the results. The LAMP detection of S. aureus was sensitive up to 1.25 CFU per reaction tube, with a detection limit of 10.3 CFU per reaction tube in the artificial contamination test, and the experiment took less than 1 h. Sensitivity of the method in Staphylococcus aureus detection was 100%, with 97.93% specificity and a 98.5% match ratio when compared using Chinese National Standard. The LAMP method is superior to the Chinese National Standard method in terms of sensitivity, cost, testing time, and convenience.

引用

页码：769 / 776

页数：8

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