Identification of new subunits of the multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase complex

被引：199

作者：

Cai, Y

Jin, JJ

Tomomori-Sato, C

Sato, S

Sorokina, I

Parmely, TJ

Conaway, RC

Conaway, JW

机构：

[1] Stowers Inst Med Res, Kansas City, MO 64110 USA

[2] Univ Kansas, Med Ctr, Dept Biochem & Mol Biol, Kansas City, KS 66160 USA

[3] Univ Oklahoma, Hlth Sci Ctr, Dept Biochem & Mol Biol, Oklahoma City, OK 73190 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2003年 / 278卷 / 44期

关键词：

D O I：

10.1074/jbc.C300389200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The mammalian ATM/PI 3-kinase-related TRRAP protein was previously found to be a component of a multiprotein histone acetyltransferase ( HAT) complex containing the HAT TIP60. In this report, we identify a previously uncharacterized protein encoded by the FLJ10914 ORF, which we designate MRGBP, as a new component of the TRRAP/TIP60 HAT complex. In addition, through purification of MRGBP and its associated proteins from HeLa cell nuclear extracts, we identify the thyroid receptor coactivating protein (TRCp120), DMAP1, and the related MRG15 and MRGX proteins as MRGBP-associating proteins, and we present biochemical evidence that they are previously unrecognized components of the TRRAP/TIP60 HAT complex. Taken together, our findings shed new light on the structure and function of the mammalian TRRAP/TIP60 histone acetyltransferase complex.

引用

收藏

页码：42733 / 42736

页数：4

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