Reconstitution of yeast RNA polymerase I transcription in vitro from purified components -: TATA binding protein is not required for basal transcription

被引：92

作者：

Keener, J ^{[1
]}

Josaitis, CA ^{[1
]}

Dodd, JA ^{[1
]}

Nomura, M ^{[1
]}

机构：

[1] Univ Calif Irvine, Dept Biol Chem, Irvine, CA 92697 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1998年 / 273卷 / 50期

关键词：

D O I：

10.1074/jbc.273.50.33795

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Five purified protein components, RNA polymerase I, Rrn3p, core factor, TBP (TATA-binding protein), and upstream activation factor, are sufficient for high level transcription in vitro from the Saccharomyces cerevisiae rDNA promoter. Rrn3p and pol I form a complex in solution that is active in specific initiation. Three protein components, pol I, Rrn3p, and core factor, and promoter sequence to -38, suffice for basal transcription. Unlike pol II and pol III, yeast pol I basal transcription does not require TBP. Instead, TBP, upstream activation factor, and the upstream. element of the promoter together stimulate pol I basal transcription to a fully activated level. The role of TBP in pol I transcription is fundamentally different from its role in pol II or pol III transcription.

引用

页码：33795 / 33802

页数：8

共 45 条

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