Effects of growth temperature on the fatty acid composition of the free-living nematode Caenorhabditis elegans

被引：97

作者：

Tanaka, T ^{[1
]}

Ikita, K ^{[1
]}

Ashida, T ^{[1
]}

Motoyama, Y ^{[1
]}

Yamaguchi, Y ^{[1
]}

Satouchi, K ^{[1
]}

机构：

[1] FUKUYAMA UNIV,DEPT BIOTECHNOL,FUKUYAMA,HIROSHIMA 72902,JAPAN

来源：

LIPIDS | 1996年 / 31卷 / 11期

关键词：

D O I：

10.1007/BF02524292

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of growth temperature on the fatty acid compositions of the phosphatidylcholine (PC), phosphatidylethanolamine (PE), and total lipid (TL) fractions of the free-living nematode Caenorhabditis elegans were investigated. A reduction in growth temperature from 25 to 15 degrees C caused the proportions of eicosapentaenoic acid (20:5n-3) to increase from 23.6 to 32.5% in the PC, from 7.4 to 10.8% in the PE, and from 12.9 to 19.9% in the TL fractions. Conversely, the levels of dihomo-gamma-linolenic acid (20:3n-6) and arachidonic acid (20:4n-6) in these phospholipid fractions and the TL fraction both decreased with decreasing growth temperature. Analysis of the positional distribution of fatty acids in the PC fraction revealed that the change in the composition of C-20 polyunsaturated fatty acid was obvious in position sn-2. Lowering the growth temperature induced an increase in the level of the diacyl subclass of PE from 58% at 25 degrees C to 71% at 75 degrees C, with a concomitant decrease in the levels of the alkylacyl and alkenylacyl subclass of PE or C. elegans. These changes observed in the phospholipids of C. elegans might be one mechanism for adaptation to low temperature.

引用

页码：1173 / 1178

页数：6

共 21 条

[1]

BARTLETT GR, 1959, J BIOL CHEM, V234, P466

[2]

BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911

[3] BIOSYNTHESIS OF LINOLEIC-ACID IN A TERMITE, COCKROACH AND CRICKET [J].

BLOMQUIST, GJ ;

DWYER, LA ;

CHU, AJ ;

RYAN, RO ;

DERENOBALES, M .

INSECT BIOCHEMISTRY, 1982, 12 (03) :349-353

[4] STRUCTURAL ORDER OF MEMBRANES AND COMPOSITION OF PHOSPHOLIPIDS IN FISH BRAIN-CELLS DURING THERMAL ACCLIMATIZATION [J].

BUDA, C ;

DEY, I ;

BALOGH, N ;

HORVATH, LI ;

MADERSPACH, K ;

JUHASZ, M ;

YEO, YK ;

FARKAS, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (17) :8234-8238

[5] DIACYL, ALKYLACYL AND ALKENYLACYL PHOSPHOLIPIDS OF THE NEMATODE TURBATRIX-ACETI [J].

CHITWOOD, DJ ;

KRUSBERG, LR .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1981, 69 (01) :115-120

[6] THE DELTA-12-DESATURASE FROM THE HOUSE CRICKET, ACHETA-DOMESTICUS (ORTHOPTERA, GRYLLIDAE) - CHARACTERIZATION AND FORM OF THE SUBSTRATE [J].

CRIPPS, C ;

BORGESON, C ;

BLOMQUIST, GJ ;

DERENOBALES, M .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1990, 278 (01) :46-51

[7]

FODOR A, 1994, J NEMATOL, V26, P278

[8] MOLECULAR ARCHITECTURE AND BIOPHYSICAL PROPERTIES OF PHOSPHOLIPIDS DURING THERMAL ADAPTATION IN FISH - AN EXPERIMENTAL AND MODEL STUDY [J].

FODOR, E ;

JONES, RH ;

BUDA, C ;

KITAJKA, K ;

DEY, I ;

FARKAS, T .

LIPIDS, 1995, 30 (12) :1119-1126

[9] METHYL-DIRECTED DESATURATION OF ARACHIDONIC TO EICOSAPENTAENOIC ACID IN THE FUNGUS, SAPROLEGNIA-PARASITICA [J].

GELLERMAN, JL ;

SCHLENK, H .

BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 573 (01) :23-30

[10] EFFECT OF GROWTH TEMPERATURE ON THE POSITIONAL DISTRIBUTION OF EICOSAPENTAENOIC ACID AND TRANS HEXADECENOIC ACID IN THE PHOSPHOLIPIDS OF A VIBRIO SPECIES OF BACTERIUM [J].

HENDERSON, RJ ;

MILLAR, RM ;

SARGENT, JR .

LIPIDS, 1995, 30 (02) :181-185

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