A model of the ACE2 structure and function as a SARS-CoV receptor

被引:152
作者
Prabakaran, P [1 ]
Mao, XD [1 ]
Dimitrov, DS [1 ]
机构
[1] NCI, Lab Expt & Computat Biol, CCR, Frederick, MD 21702 USA
关键词
ACE2; structure prediction; SARS-CoV; S-glycoprotein; receptor; binding;
D O I
10.1016/j.bbrc.2003.12.081
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The angiotensin-converting enzyme 2 (ACE2) is an important regulator of the renin-angiotensin system and was very recently identified as a functional receptor for the SARS virus. The ACE2 sequence is similar (sequence identities 43% and 35%, and similarities 61% and 55%, respectively) to those of the testis-specific form of ACE (tACE) and the Drosophila homolog of ACE (AnCE). The high level of sequence similarity allowed us to build a robust homology model of the ACE2 structure with a root-mean-square deviation from the aligned crystal structures of tACE and AnCE less than 0.5Angstrom. A prominent feature of the model is a deep channel on the top of the molecule that contains the catalytic site. Negatively charged ridges surrounding the channel may provide a possible binding site for the positively charged receptor-binding domain (RBD) of the S-glycoprotein, which we recently identified [Biochem. Biophys. Res. Commun. 312 (2003) 1159]. Several distinct patches of hydrophobic residues at the ACE2 surface were noted at close proximity to the charged ridges that could contribute to binding. These results suggest a possible binding region for the SARS-CoV S-glycoprotein on ACE2 and could help in the design of experiments to further elucidate the structure and function of ACE2. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:235 / 241
页数:7
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