Effect of profound hypothermia on genomics of hippocampus following complete cerebral ischemia in rats

被引：10

作者：

Qin, Hua-Ping ^{[1
]}

Mei, Guang-Hai ^{[1
]}

Wei, Liang ^{[1
]}

Jiang, Ji-Yao ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Dept Neurosurg, Renji Hosp, Sch Med, Shanghai 200127, Peoples R China

来源：

NEUROLOGICAL RESEARCH | 2008年 / 30卷 / 05期

关键词：

cerebral ischemia; profound hypothermia; gene chips; genomics;

D O I：

10.1179/174313208X284250

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Objective: To determine differential gene expression of hippocampus in rats following complete cerebral ischemia with treatment of profound hypothermia compared to normothermia. Methods: Six rats got 5 minutes of complete cerebral ischemia with circulatory arrest and randomly divided into two groups: normothermia ischemia group (37 +/- 0.3 degrees C, n=3) and profound hypothermia ischemia group (18 +/- 0.5 degrees C, n=3). Affymetrix U34A rat arrays were applied to detect the difference of gene expression profile in hippocampus between the two groups. Results: Expression profiles of a total of 75 transcripts in the profound hypothermia ischemia group were statistically different from those of the normothermia ischemia group, and 33 of them were significantly up-regulated and other 42 were significantly down-regulated (p < 0.01). Conclusions: Compared with normothermia, profound hypothermia had a significant effect on the gene expression profiles following complete cerebral ischemia, which may be involved in the mechanisms of cerebral protection by profound hypothermia.

引用

页码：536 / 541

页数：6

共 31 条

[1] EMERGENCY BRAIN RESUSCITATION [J].

ALBERTS, MJ ;

LYDEN, PD ;

ZIVIN, JA ;

BROTT, TJ ;

BRASS, LM .

ANNALS OF INTERNAL MEDICINE, 1995, 122 (08) :622-627

[2] MANAGEMENT OF PROFOUND ACCIDENTAL HYPOTHERMIA WITH CARDIORESPIRATORY ARREST [J].

ALTHAUS, U ;

AEBERHARD, P ;

SCHUPBACH, P ;

NACHBUR, BH ;

MUHLEMANN, W .

ANNALS OF SURGERY, 1982, 195 (04) :492-495

[3] Gene Ontology: tool for the unification of biology [J].

Ashburner, M ;

Ball, CA ;

Blake, JA ;

Botstein, D ;

Butler, H ;

Cherry, JM ;

Davis, AP ;

Dolinski, K ;

Dwight, SS ;

Eppig, JT ;

Harris, MA ;

Hill, DP ;

Issel-Tarver, L ;

Kasarskis, A ;

Lewis, S ;

Matese, JC ;

Richardson, JE ;

Ringwald, M ;

Rubin, GM ;

Sherlock, G .

NATURE GENETICS, 2000, 25 (01) :25-29

[4] HYPOTHERMIA - ITS POSSIBLE ROLE IN CARDIAC SURGERY - AN INVESTIGATION OF FACTORS GOVERNING SURVIVAL IN DOGS AT LOW BODY TEMPERATURES [J].

BIGELOW, WG ;

LINDSAY, WK ;

GREENWOOD, WF .

ANNALS OF SURGERY, 1950, 132 (05) :849-866

[5] EFFECT OF MILD HYPOTHERMIA ON ISCHEMIA-INDUCED RELEASE OF NEUROTRANSMITTERS AND FREE FATTY-ACIDS IN RAT-BRAIN [J].

BUSTO, R ;

GLOBUS, MY ;

DIETRICH, WD ;

MARTINEZ, E ;

VALDES, I ;

GINSBERG, MD .

STROKE, 1989, 20 (07) :904-910

[6]

CHURN SB, 1999, STROKE, V21, P758

[7] MARKED PROTECTION BY MODERATE HYPOTHERMIA AFTER EXPERIMENTAL TRAUMATIC BRAIN INJURY [J].

CLIFTON, GL ;

JIANG, JY ;

LYETH, BG ;

JENKINS, LW ;

HAMM, RJ ;

HAYES, RL .

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1991, 11 (01) :114-121

[8]

GINSBERG MD, 1992, CEREBROVAS BRAIN MET, V4, P189

[9] CEREBRAL CONSEQUENCES OF HYPOTHERMIC CIRCULATORY ARREST IN ADULTS [J].

GRIEPP, EB ;

GRIEPP, RB .

JOURNAL OF CARDIAC SURGERY, 1992, 7 (02) :134-155

[10] Neurological injury during cardiopulmonary bypass in the rat [J].

Grocott, HP ;

Mackensen, GB ;

Newman, MF ;

Warner, DS .

PERFUSION-UK, 2001, 16 (01) :75-81

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