A defect in glyoxysomal fatty acid β-oxidation reduces jasmonic acid accumulation in Arabidopsis

被引:49
作者
Afitlhile, MM
Fukushige, H
Nishimura, M
Hildebrand, DF
机构
[1] Univ Kentucky, Dept Agron, Lab Plant Biochem & Genet, Lexington, KY 40546 USA
[2] Kentucky State Univ, Land Grant Program, CRS, Atwood Res Facil, Frankfort, KY 40601 USA
[3] Natl Inst Basic Biol, Dept Cell Biol, Okazaki, Aichi 4448585, Japan
关键词
Arabidopsis; jasmonic acid; 12-oxo-phytodienoic acid; OPC-8 : 0; beta-oxidation; ped1; thiolase;
D O I
10.1016/j.plaphy.2005.03.016
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The final steps of jasmonic acid (JA) biosynthesis are thought to involve peroxisomal (3-oxidation, but this has not been directly demonstrated. The last and key step in fatty acid (3-oxidation is catalyzed by 3-ketoacyl-CoA thiolase (KAT) (EC 2.3.1.16). A mutant of Arabidopsis thaliana ecotype Landsberg erecta, which lacks a functional KAT protein and is defective in glyoxysomal fatty acid (3-oxidation has been reported. In this study, the mutant was found to accumulate reduced level of JA in both its wounded cotyledons and leaves, while only the cotyledons accumulate 3-oxo-2-(pent-2'-enyl)-cyclopentane-1-octanoic acid (OPC-8:0). This indicates that a defect in one of the thiolase isoenzymes impairs (3-oxidation of OPC-8:0 to JA. The mutant had sufficient thiolase activity for the synthesis of JA in the unwounded but not in the wounded tissues. Activities of the enzymes in the JA pathway that catalyze the steps, which precede (3-oxidation were not altered by the mutation in a thiolase protein. Thus, reduced levels of JA in the wounded tissues of the mutant were attributed to the defect in a thiolase protein. (c) 2005 Elsevier SAS. All rights reserved
引用
收藏
页码:603 / 609
页数:7
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