Transgenic elite Indica rice varieties, resistant to Xanthomonas oryzae pv. oryzae

被引:54
作者
Zhang, SP
Song, WY
Chen, LL
Ruan, DL
Taylor, N
Ronald, P
Beachy, R
Fauquet, C
机构
[1] Scripps Res Inst, Div Plant Biol BCC 206, TSRI, ORSTOM,Int Lab Trop Agr Biotechnol, La Jolla, CA 92037 USA
[2] Univ Calif Davis, Davis, CA 95616 USA
关键词
Indica rice; cell suspension; transformation; Xa21; bacterial leaf blight;
D O I
10.1023/A:1009601520901
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The agronomically important Indica (group 1) rice varieties IR64, IR72, hybrid restorer line Minghui 63, and BG90-2 were co-transformed by microbombardment of embryogenic suspensions with plasmids that contain the Xa21 gene which confers resistance to Xanthomonas oryzae pv. oryzae and the hph gene for resistance to hygromycin B. Six of the 55 transgenic R0 plant lines containing the Xa21 gene displayed high levels of resistance to the pathogen, and no partial resistance was observed. The trait was stably inherited in subsequent generations, and transgenic plants are currently in field tests. The ability to transfer agronomically important genes into elite Indica rice varieties demonstrates the applicability of genetic engineering for the agronomic improvement of rice.
引用
收藏
页码:551 / 558
页数:8
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