Expression of attractin and its differential enzyme activity in glioma cells

被引：16

作者：

Malík, R

Mares, V

Kleibl, Z

Pohlreich, P

Vlasicová, K

Sedo, A

机构：

[1] First Fac Med, Joint Lab Canc Cell Biol, Dept Med Chem & Biochem 1, CZ-12108 Prague 2, Czech Republic

[2] First Fac Med, Joint Lab Canc Cell Biol, Dept Med Chem & Biochem 2, CZ-12108 Prague 2, Czech Republic

[3] Acad Sci Czech Republic, Inst Physiol, CZ-14220 Prague 4, Czech Republic

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 284卷 / 02期

关键词：

glioma; attractin; dipeptidyl peptidase; CD26; cell transformation;

D O I：

10.1006/bbrc.2001.4956

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Attractin/mahogany protein was previously shown to be involved in a number of physiological and pathological events, including immune system regulation, body weight control, pigmentation, myelinization, and tumor susceptibility. Human attractin has an enzymatic activity resembling dipeptidyl peptidase IV (DPP-IV). In the central nervous system, attractin has been detected in neurons but not in glial, cells up to now. We show the expression of attractin mRNA and protein in glioma cell lines at different degree of transformation. In human U373 and U87 glioma cells (Grades III and TV), membrane-bound attractin displays hydrolytic activity amounting to 5 and 25% of total cellular DPP-TV-like enzyme activity, respectively. Such activity has not been observed in the rat Cg glioma cells (Grade I). Attractin presence in glioma, but not in normal glial cells, together with its differential enzymatic activity, suggests its role in growth properties of tumors of glial cell origin, (C) 2001 Academic Press.

引用

页码：289 / 294

页数：6