Reconstitution of Sec-dependent membrane protein insertion: nascent FtsQ interacts with YidC in a SecYEG-dependent manner

被引：98

作者：

van der Laan, M

Houben, ENG

Nouwen, N

Luirink, J

Driessen, AJM

机构：

[1] Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Dept Microbiol, NL-9751 NN Haren, Netherlands

[2] Bioctr Amsterdam, Inst Mol Biol Sci, Dept Microbiol, NL-1081 HV Amsterdam, Netherlands

来源：

EMBO REPORTS | 2001年 / 2卷 / 06期

关键词：

D O I：

10.1093/embo-reports/kve106

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The inner membrane protein YidC is associated with the preprotein translocase of Escherichia coli and contacts transmembrane segments of nascent inner membrane proteins during membrane insertion. YidC was purified to homogeneity and co-reconstituted with the SecYEG complex. YidC had no effect on the SecA/SecYEG-mediated translocation of the secretory protein proOmpA; however, using a crosslinking approach, the transmembrane segment of nascent FtsQ was found to gain access to YidC via SecY. These data indicate the functional reconstitution of the initial stages of YidC-dependent membrane protein insertion via the SecYEG complex.

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收藏

页码：519 / 523

页数：5

相关论文

共 28 条

[11] Cysteine-directed cross-linking demonstrates that helix 3 of SecE is close to helix 2 of SecY and helix 3 of a neighboring SecE [J].

Kaufmann, A ;

Manting, EH ;

Veenendaal, AKJ ;

Driessen, AJM ;

van der Does, C .

BIOCHEMISTRY, 1999, 38 (28) :9115-9125

[12]

LEE JI, 1992, J BIOL CHEM, V267, P938

[13] SECA PROTEIN HYDROLYZES ATP AND IS AN ESSENTIAL COMPONENT OF THE PROTEIN TRANSLOCATION ATPASE OF ESCHERICHIA-COLI [J].

LILL, R ;

CUNNINGHAM, K ;

BRUNDAGE, LA ;

ITO, K ;

OLIVER, D ;

WICKNER, W .

EMBO JOURNAL, 1989, 8 (03) :961-966

[14] AN ALTERNATIVE PROTEIN TARGETING PATHWAY IN ESCHERICHIA-COLI - STUDIES ON THE ROLE OF FTSY [J].

LUIRINK, J ;

TENHAGENJONGMAN, CM ;

VANDERWEIJDEN, CC ;

OUDEGA, B ;

HIGH, S ;

DOBBERSTEIN, B ;

KUSTERS, R .

EMBO JOURNAL, 1994, 13 (10) :2289-2296

[15] SecYEG assembles into a tetramer to form the active protein translocation channel [J].

Manting, EH ;

van der Does, C ;

Remigy, H ;

Engel, A ;

Driessen, AJM .

EMBO JOURNAL, 2000, 19 (05) :852-861

[16] Escherichia coli translocase:: the unravelling of a molecular machine [J].

Manting, EH ;

Driessen, AJM .

MOLECULAR MICROBIOLOGY, 2000, 37 (02) :226-238

[17] SRP-dependent co-translational targeting and SecA-dependent translocation analyzed as individual steps in the export of a bacterial protein [J].

Neumann-Haefelin, C ;

Schäfer, U ;

Müller, W ;

Koch, HG .

EMBO JOURNAL, 2000, 19 (23) :6419-6426

[18] Evolutionarily conserved binding of ribosomes to the translocation channel via the large ribosomal RNA [J].

Prinz, A ;

Behrens, C ;

Rapoport, TA ;

Hartmann, E ;

Kalies, KU .

EMBO JOURNAL, 2000, 19 (08) :1900-1906

[19] YidC mediates membrane protein insertion in bacteria [J].

Samuelson, JC ;

Chen, MY ;

Jiang, FL ;

Möller, I ;

Wiedmann, M ;

Kuhn, A ;

Phillips, GJ ;

Dalbey, RE .

NATURE, 2000, 406 (6796) :637-641

[20] BLUE NATIVE ELECTROPHORESIS FOR ISOLATION OF MEMBRANE-PROTEIN COMPLEXES IN ENZYMATICALLY ACTIVE FORM [J].

SCHAGGER, H ;

VONJAGOW, G .

ANALYTICAL BIOCHEMISTRY, 1991, 199 (02) :223-231