microRNA detection comes of age

被引:42
作者
Hammond, SM [1 ]
机构
[1] Univ N Carolina, Lineberger Comprehens Canc Ctr, Dept Cell & Dev Biol, Chapel Hill, NC 27599 USA
关键词
D O I
10.1038/nmeth0106-12
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The short nature of microRNAs (miRNAs) has presented unique obstacles to experimental biologists. Two research papers in this issue of Nature Methods describe solutions to some of these problems and provide high-resolution data on the expression patterns of these tiny regulatory RNAs.
引用
收藏
页码:12 / 13
页数:2
相关论文
共 9 条
[1]   MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].
Bartel, David P. .
CELL, 2007, 131 (04) :11-29
[2]   MUTATIONS THAT LEAD TO REITERATIONS IN THE CELL LINEAGES OF C-ELEGANS [J].
CHALFIE, M ;
HORVITZ, HR ;
SULSTON, JE .
CELL, 1981, 24 (01) :59-69
[3]  
Kim VN, 2005, MOL CELLS, V19, P1
[4]   In situ detection of miRNAs in animal embryos using LNA-modified oligonucteotide probes [J].
Kloosterman, WP ;
Wienholds, E ;
de Bruijn, E ;
Kauppinen, S ;
Plasterk, RHA .
NATURE METHODS, 2006, 3 (01) :27-29
[5]   Identification of tissue-specific microRNAs from mouse [J].
Lagos-Quintana, M ;
Rauhut, R ;
Yalcin, A ;
Meyer, J ;
Lendeckel, W ;
Tuschl, T .
CURRENT BIOLOGY, 2002, 12 (09) :735-739
[6]   THE C-ELEGANS HETEROCHRONIC GENE LIN-4 ENCODES SMALL RNAS WITH ANTISENSE COMPLEMENTARITY TO LIN-14 [J].
LEE, RC ;
FEINBAUM, RL ;
AMBROS, V .
CELL, 1993, 75 (05) :843-854
[7]   A single-molecule method for the quantitation of microRNA gene expression [J].
Neely, LA ;
Patel, S ;
Garver, J ;
Gallo, M ;
Hackett, M ;
McLaughlin, S ;
Nadel, M ;
Harris, J ;
Gullans, S ;
Rooke, J .
NATURE METHODS, 2006, 3 (01) :41-46
[8]   Microarray-based, high-throughput gene expression profiling of microRNAs [J].
Nelson, PT ;
Baldwin, DA ;
Scearce, LM ;
Oberholtzer, JC ;
Tobias, JW ;
Mourelatos, Z .
NATURE METHODS, 2004, 1 (02) :155-161
[9]   A custom microarray platform for analysis of microRNA gene expression [J].
Thomson, JM ;
Parker, J ;
Perou, CM ;
Hammond, SM .
NATURE METHODS, 2004, 1 (01) :47-53