Ultrasound-assited extraction and structural identification of polysaccharides from Isodon lophanthoides var. gerardianus (Bentham) H. Hara

被引：75

作者：

Wen, Lingrong ^{[1
]}

Lin, Lianzhu ^{[1
]}

You, Lijun ^{[1
]}

Yang, Bao ^{[2
]}

Jiang, Guoxiang ^{[2
]}

Zhao, Mouming ^{[1
]}

机构：

[1] S China Univ Technol, Coll Light Ind & Food Sci, Guangzhou 510640, Peoples R China

[2] Chinese Acad Sci, S China Bot Garden, Key Lab Plant Resource Conservat & Sustainable Ut, Guangzhou 510650, Guangdong, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2011年 / 85卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Isodon lophanthoides var. gerardianus; Polysaccharides; Ultrasound; Structural identification; Antioxidant activity; RESPONSE-SURFACE METHODOLOGY; LONGAN FRUIT PERICARP; ASSISTED EXTRACTION; ANTIOXIDANT ACTIVITY; RABDOSIA-LOPHANTHOIDES; MORINDA-CITRIFOLIA; ABIETANE QUINONES; OPTIMIZATION; OLIGOSACCHARIDES; ANTHRAQUINONES;

D O I：

10.1016/j.carbpol.2011.03.003

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Ultrasound-assisted extraction was employed to prepare polysaccharides from Isodon lophanthoides var. gerardianus (Bentham) H. Hara (Xihuangcao). Response surface methodology was used to optimize the ultrasound-assisted extraction parameters. Central-composite design was applied to estimate the effects of ultrasonic time, liquid/solid ratio and pH on the yield of the polysaccharides from I. lophanthoides var. gerardianus (Bentham) H. Hara (ILHP). A mathematical model with high fitness was obtained. Ultrasonic time, liquid/solid ratio and pH exhibited independent and interactive effects on ILHP yield. Gas chromatography analysis suggested that ILHP-3 comprised Rha, Man, Glc with relative molar percentages of 3.9%, 8.0% and 88.1%, respectively. The assay of glycosidic linkage showed that ILHP-3 was consisted of -> 2)-Man-(1 ->, -> 6)-Glc-(1 ->, -> 3)-Rha-(1 -> with a molar proportion of 1.9:26.5:1. The average molecular weight of ILHP-3 was measured to be 247 kDa by high-performance gel permeation chromatography. Furthermore, a good antioxidant activity was observed for ILHP-3. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：541 / 547

页数：7

共 36 条

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